Probe:	2C-3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Probe hydrolyzed to an average size of 150 bp.

Structure	Level	Pattern	Image	Note
TS9: extraembryonic visceral endoderm	Present
TS9: embryo	Absent			Expression was absent in the embryo proper.

Structure	Level	Pattern	Image	Note
TS10: extraembryonic visceral endoderm	Present
TS10: embryo	Absent			Expression was absent in the embryo proper.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present
TS11: embryo ectoderm	Absent

Structure	Level	Pattern	Image	Note
TS12: extraembryonic visceral endoderm	Present			Expression has declined from TS 10.
TS12: ectoplacental cone	Strong	Regionally restricted		Strong expression in the large cells of the ectoplacental cone.
TS12: embryo mesenchyme	Present	Uniform
TS12: embryo ectoderm	Absent

Structure	Level	Pattern	Image	Note
TS14: embryo mesenchyme	Present
TS14: head somite	Present
TS14: trunk somite	Present
TS14: heart	Present	Regionally restricted		Expression is absent in much of the wall of the developing heart.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted		Expression is present in the most dorsal portion of the tube.
TS15: embryo mesenchyme	Present
TS15: trunk somite	Present
TS15: heart	Present	Regionally restricted		Expression is absent in much of the wall of the developing heart.

Structure	Level	Pattern	Image	Note
TS16: trunk somite	Present		2	Expression has declined compared to TS 14.
TS16: trunk dermomyotome	Present	Regionally restricted	2	Expression is present in the early dermatomes; the late ones are unlabeled, as are the myotomes.
TS16: trunk sclerotome	Strong		2
TS16: mesenchyme derived from splanchnopleure	Present		2	Expression in the splanchnic mesodermal plate.
TS16: embryo mesenchyme	Present	Regionally restricted	2	Expression is present in mesenchyme surrounding the neural tube, the somites, the developing vasculature, mesenchyme condensing to form major blood vessels, as well as in mesodermal mesenchyme. Expression is absent from future muscle.
TS16: future hindbrain roof plate	Present		2	Expression is present in the roof.

Structure	Level	Pattern	Image	Note
TS16: peripheral nervous system	Absent			Expression is absent from the proximal trigeminal, sensory, and sympathetic ganglia.
TS16: cardiovascular system	Absent			There is no expression in the endothelium.
TS16: gut	Absent			There is no expression in definitive endodermal derivatives.
TS16: embryo ectoderm	Absent			There is no expression in most ectodermal derivatives.

Structure	Level	Pattern	Image	Note
TS18: embryo	Strong	Regionally restricted		Expression resembles that seen at E10.0.

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Strong		4A
TS19: embryo mesenchyme	Strong		4A	Expression is present in mesenchyme derived from mesoderm.
TS19: left atrium auricular region	Present	Regionally restricted	4A	Expression is present in small sections of the myocardium. There is no expression in the endocardium.
TS19: right atrium auricular region	Present	Regionally restricted	4A	Expression is present in small sections of the myocardium. There is no expression in the endocardium.
TS19: heart	Present	Regionally restricted	4A	Expression is present in the primitive trabeculae.
TS19: atrioventricular canal	Present	Regionally restricted	4A	Expression is present in the cushion tissue.
TS19: outflow tract	Present	Regionally restricted	4A	Expression is present in the cushion tissue of the truncus.
TS19: pericardial component	Present		4A

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Strong		9	Expression is present within the epithelial cells that are forming the lens of the eye.
TS19: retina	Absent		9	There is no expression within the optic cup.

Structure	Level	Pattern	Image	Note
TS19: heart	Present	Regionally restricted		Although there is expression elsewhere in the heart, there in none in the endocardium.
TS19: peripheral nervous system	Absent			There is no expression in the neural crest-derived proximal trigeminal, sensory, and sympathetic ganglia.
TS19: gut	Absent			There is no expression in the definitive endodermal derivatives.
TS19: embryo	Present	Regionally restricted		Although there is expression elsewhere in the embryo, there is no expression in muscle nor in endothelium.

Structure	Level	Pattern	Image	Note
TS21: embryo mesenchyme	Present	Graded	5	Expression is present throughout the mesenchyme but is particularly intense where mesenchyme is surrounding developing cartilage and bone; around blood vessels; and where mesenchyme and epithelium are interacting to form new organs.
TS21: tail sclerotome	Strong		5	Expression is strong in the sclerotomes. The condrocytic core of the cartilage is losing expression.
TS21: axial skeleton	Strong	Regionally restricted	5	Expression is strong within the perichondrium and periosteum of the developing bones. There is no expression in the osteoblasts.
TS21: heart	Present	Regionally restricted	5	Some expression is present in the ventricular trabeculae and in a minority of the myocardium. No expression is present within the endocardium.
TS21: pericardium	Present		5
TS21: dermis	Present		5
TS21: blood vessel	Absent		5	There is no expression in the endothelium.

Structure	Level	Pattern	Image	Note
TS21: peripheral nervous system	Absent			There is no expression in neural crest-derived proximal trigeminal, sensory, and sympathetic ganglia.
TS21: brain	Present	Patchy
TS21: cerebellum	Present	Patchy
TS21: medulla oblongata	Present	Patchy
TS21: midbrain	Present	Patchy
TS21: pons	Present	Patchy
TS21: spinal cord	Present	Patchy
TS21: gut	Absent			There is no expression in the definitive endodermal derivatives nor in muscle.
TS21: skeletal muscle	Absent			There is no expression in the definitive endodermal derivatives nor in muscle.

Structure	Level	Pattern	Image	Note
TS23: heart valve	Strong
TS23: heart	Present	Regionally restricted		There are small pockets of labeling in the myocardium.
TS23: peripheral nervous system	Absent			There is no expression in neural crest-derived proximal trigeminal, sensory, and sympathetic ganglia.
TS23: embryo mesenchyme	Present	Graded		Expression is present throughout the mesenchyme but is particularly intense where mesenchyme is surrounding developing cartilage and bone; around blood vessels; and where mesenchyme and epithelium are interacting to form new organs.