Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Calcatm1Eme
Name:	calcitonin/calcitonin-related polypeptide, alpha; targeted mutation 1, Ronald Emeson
MGI ID:	MGI:2148538
Synonyms:	alpha-CGRP-, alphaCGRP-, Calcatm1Jrs, CGRP -
Gene:	Calca  Location: Chr7:114230713-114235592 bp, - strand  Genetic Position: Chr7, 59.99 cM
Alliance:	Calcatm1Eme page

Germline Transmission:	Earliest citation of germline transmission: J:57708
Parent Cell Line:	TL1/TL-1 (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted (Modified isoform(s), Null/knockout) Mutation:    Nucleotide substitutions   Mutation details: A mutation was introduced to create a stop codon immediately upstream of the coding region of the CGRP alternative splice transcript in exon 5. The codons for a 4-amino-acid peptide (DLQA) of unknown function that is normally proteolytically spliced from the C-terminus of the alphaCGRP prohormone, was duplicated and inserted after the codons for a dibasic proteolytic cleavage site (normally used to cleave CGRP from the alphaCGRP prohormone) and before the stop codon in order to preserve the expression of this peptide. Thus, only the CGRP expression is knocked-out and the expression of the other neuropeptides created from this locus (calcitonin, etc.) is left undisturbed. (J:57708)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Calca Mutation:	21 strains or lines available

Original:	J:57708 Lu JT, et al., Mice lacking alpha-calcitonin gene-related peptide exhibit normal cardiovascular regulation and neuromuscular development. Mol Cell Neurosci. 1999 Aug;14(2):99-120
All:	16 reference(s)