Gene Expression Data
Assay Details
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Assay
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Reference:
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J:70745
Suda Y, et al., Emx2 directs the development of diencephalon in cooperation with Otx2. Development. 2001 Jul;128(13):2433-50
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2148283
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Gene symbol:
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Wnt1
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Gene name:
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wingless-type MMTV integration site family, member 1
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Probe:
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Wnt1 probe3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
3G:
embryonic day 9.5
(more  )
Specimen
3G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: diencephalon |
Present |
Regionally restricted |
3G
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Expression in the dorsal midline over the caudal diencephalon.
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| TS15: future hindbrain |
Present |
Regionally restricted |
3G
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Expression in the dorsal midline over the mesencephalon and in a characteristic lateral stripe at the isthmus.
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| TS15: future midbrain |
Present |
Regionally restricted |
3G
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Expression in the dorsal midline over the mesencephalon and in a characteristic lateral stripe at the isthmus.
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| TS15: rhombomere 1 |
Absent |
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3G
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Caudal to isthmus, no expression in the anterior metencephalon, rhombomeres 1 and 2 (r1 and r2).
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| TS15: rhombomere 2 |
Absent |
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3G
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Caudal to isthmus, no expression in the anterior metencephalon, rhombomeres 1 and 2 (r1 and r2).
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| TS15: future spinal cord |
Present |
Regionally restricted |
3G
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Anterior terminus of expression in roof of spinal cord at rhombomere 3 (r3).
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| TS15: rhombomere 3 |
Present |
Regionally restricted |
3G
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Anterior terminus of expression in roof of spinal cord at rhombomere 3 (r3).
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Specimen
3H:
embryonic day 9.5; Emx2tm1Sia/ Emx2tm1Sia, Otx2tm1Sia/Otx2 +
(more )
Note: Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 and Otx2 heterozygotes (from F3 and F5 generations, respectively) were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Specimen
3H:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 and Otx2 heterozygotes (from F3 and F5 generations, respectively) were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: diencephalon |
Present |
Regionally restricted |
3H
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Reduced region of expression in the roof; decrease in level of expression laterally.
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| TS15: future hindbrain |
Present |
Regionally restricted |
3H
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Reduced region of expression in the roof; decrease in level of expression laterally; isthmic stripe shifted rostrally.
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| TS15: future midbrain |
Present |
Regionally restricted |
3H
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Reduced region of expression in the roof; decrease in level of expression laterally; isthmic stripe shifted rostrally.
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| TS15: rhombomere 1 |
Absent |
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3H
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The Wnt1-negative region is expanded in the double mutants.
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| TS15: rhombomere 2 |
Absent |
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3H
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The Wnt1-negative region is expanded in the double mutants.
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Specimen
4E:
embryonic day 8.0
(more )
Note: 6-somite stage.
Specimen
4E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6-somite stage.
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Specimen
4F:
embryonic day 8.0; Emx2tm1Sia/ Emx2tm1Sia, Otx2tm1Sia/Otx2 +
(more )
Note: 6-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Specimen
4F:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
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4F
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Expression is reduced and shifted anteriorly in double mutant.
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| TS12: future hindbrain |
Absent |
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4F
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Expansion in the Wnt1-negative anterior hindbrain, corresponding to future rhombomeres 1 and 2 (r1 and r2).
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Specimen
not shown:
embryonic day 8.0; Emx2tm1Sia/ Emx2tm1Sia, Otx2tm1Sia/Otx2 +
(more )
Note: 4-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Specimen
not shown:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Weak |
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Authors state that Wnt1 expression is faint in double mutants at the 4-somite stage.
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Specimen
8A:
embryonic day 10.5
(more )
Specimen
8A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Analysis Tools