Antibody:	anti-C-Isl-1 protein (K5)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	6C , 6D , 6B	Present in the dorsal region of cervical cord segments 4-8 and toracic cord segment 1.
TS19: neural tube basal columns	Present	Regionally restricted	6B , 6C , 6D	Present in the motor neurons of the ventral horn.
TS19: neural tube ventricular layer	Absent		6B , 6C , 6D	There is no expression in the intermediate part of the ependymal zone.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	6G , 6F , 6H	Present in the dorsal region of cervical cord segments 4-8 and toracic cord segment 1; there are more cells expressing the protein than in the wild type mice.
TS19: neural tube basal columns	Present	Regionally restricted	6F , 6G , 6H	Present in the motor neurons of the ventral horn.
TS19: neural tube ventricular layer	Present	Regionally restricted	6F , 6H , 6G	Present in the intermediate part of the ependymal zone.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	6J , 6K , 6L	Present in the dorsal region of cervical cord segments 4-8 and toracic cord segment 1; there are more cells expressing the protein than in the wild type mice.
TS19: neural tube basal columns	Present	Regionally restricted	6K , 6J , 6L	Present in the motor neurons of the ventral horn.
TS19: neural tube ventricular layer	Present	Regionally restricted	6K , 6J , 6L	Present in the intermediate part of the ependymal zone.