Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | Notes | References

Symbol: Apoetm1Bres Name: apolipoprotein E; targeted mutation 1, Jan L Breslow MGI ID: MGI:2137814 Synonyms: apo E-, apoE-, EKO, epsilon- Gene: Apoe  Location: Chr7:19430034-19433113 bp, - strand  Genetic Position: Chr7, 9.94 cM Alliance: Apoetm1Bres page

Gross appearance of aortic arches of Apoetm1Bres/Apoetm1Bres, Plgtm1Jld/Plgtm1Jld and Apoetm1Bres/Apoetm1Bres Plgtm1Jld/Plgtm1Jld mice Show the 3 phenotype image(s) involving this allele.

Germline Transmission:	Earliest citation of germline transmission: J:2809
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A neomycin resistance cassette replaced 1.4kb of the gene, including part of the promoter through part of the second intron. Northern blot analysis using a mouse exon 4 riboprobe did not detect wild-type message in livers from homozygous mutant mice, but did detect a longer transcript that the authors propose is a neo-Apoe fusion product. Gradient SDS-PAGE analysis did not detect full size Apoe associated with any fractions from plasma lipoproteins from homozygous mutant mice. (J:2809)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

4 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 11 strains available      Cell Lines: 0 lines available
Carrying any Apoe Mutation:	145 strains or lines available

Original:	J:2809 Plump AS, et al., Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells. Cell. 1992 Oct 16;71(2):343-53
All:	135 reference(s)