Gene Expression Data
Assay Details
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Assay
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Reference:
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J:40605
Miyamoto N, et al., Defects of urogenital development in mice lacking Emx2. Development. 1997 May;124(9):1653-64
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2136469
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Gene symbol:
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Pax2
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Gene name:
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paired box 2
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Probe:
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Pax2 cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
5A:
embryonic day 10.5
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5B:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: nephric duct |
Present |
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5B
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Expression is apparently normal in the mutant Wolffian duct and mesonephric tubules.
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| TS17: mesonephric tubule |
Present |
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5B
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Expression is apparently normal in the mutant Wolffian duct and mesonephric tubules.
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Specimen
5C:
embryonic day 11.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5F:
embryonic day 11.5
(more )
Specimen
5F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: metanephric mesenchyme |
Present |
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5F
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Expression is first observed in the metanephric mesenchyme when the ureteric bud invades.
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Specimen
5D:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: nephric duct |
Present |
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5D
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Expression in the mutant Wolffian duct.
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| TS19: mesonephric tubule |
Present |
Regionally restricted |
5D
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Expression in mesonephric tubules, but lost in several portions even though they are morphologically normal.
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| TS19: neural tube |
Present |
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5D
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Authors state that expression in the neural tube was unaffected.
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Specimen
5E:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5G:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia
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Age:
|
embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: mesenchyme of metanephros |
Present |
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5G
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In the mutant metanephros, expression is apparently normal in mesenchyme.
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| TS19: ureteric bud |
Weak |
Regionally restricted |
5G
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Expression is greatly reduced in the ureteric bud (faint in the tip of the bud, lost in the proximal part).
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Specimen
4K:
embryonic day 13.5
(more )
Specimen
4K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: metanephros |
Present |
Regionally restricted |
4K
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Continuously expressed in condensed mesenchyme and epithelializing mesenchyme.
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| TS21: ureter |
Present |
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4K
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Expression in the branched ureters.
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Analysis Tools