Summary | Mutation origin | Mutation description | Expression | Phenotypes | Disease models | Find Mice (IMSR) | References

Symbol:	Klc1tm1Gsn
Name:	kinesin light chain 1; targeted mutation 1, Lawrence S B Goldstein
MGI ID:	MGI:2136259
Gene:	Klc1  Location: Chr12:111725283-111774278 bp, + strand  Genetic Position: Chr12, 61.13 cM
Alliance:	Klc1tm1Gsn page

Germline Transmission:	Earliest citation of germline transmission: J:57877
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout, Reporter) Mutations:    Insertion, Intragenic deletion   Mutation details: An IRES Beta-geo cassette replaced the exon that encodes the entire first TPR domain and part of the second TPR domain. Western analysis of crude cytoplasmic brain extracts of homozygous mutant mice detected dramatically reduced levels of full length KLC1, as well as very small amounts of truncated KLC1. Immunoprecipitation studies did not detect either form of KLC1 in KIF5A or KIF5B coprecipitates of brain extracts of homozygous mutant mice. Immunofluorescence studies of sensory and motor neuron cell bodies in dorsal root ganglion and spinal cord of homozygous mutant mice detected very faint KCL1 staining. (J:57877)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

3 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Klc1 Mutation:	54 strains or lines available

Original:	J:57877 Rahman A, et al., Defective kinesin heavy chain behavior in mouse kinesin light chain mutants. J Cell Biol. 1999 Sep 20;146(6):1277-88
All:	10 reference(s)