Gene Expression Data
Assay Details
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Assay
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Reference:
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J:69847
Mahlapuu M, et al., Haploinsufficiency of the forkhead gene Foxf1, a target for sonic hedgehog signaling, causes lung and foregut malformations. Development. 2001 Jun;128(12):2397-406
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2135979
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Gene symbol:
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Acta2
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Gene name:
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actin alpha 2, smooth muscle, aorta
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Probe:
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IMAGE clone 1224519
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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The Foxf1a<tm1Pca> allele was maintained on mixed 129/Ola-C57BL/6 and 129/Ola-CD1 and 129/Sv-CD1 and 129/Sv-C57BL/6 backgrounds. Chimeras were mated to CD-1 mice to obtain heterozygotes.
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Results
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Specimen
1 L, +/-:
embryonic day 18.5; Foxf1tm1Pca/Foxf1 +
(more  )
Specimen
1 L, +/-:
(close  )
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Genetic Background:
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either: (involves: 129 * C57BL/6) or (involves: 129 * CD-1)
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Mutant Allele(s):
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Foxf1tm1Pca/Foxf1+
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: left lung vascular element |
Present |
Regionally restricted |
1L
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Staining of vascular smooth muscle cells. A lower level of expression in the heterozygote compared to wild-type.
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| TS26: right lung vascular element |
Present |
Regionally restricted |
1L
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Staining of vascular smooth muscle cells. A lower level of expression in the heterozygote compared to wild-type.
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Specimen
1 N, +/+:
embryonic day 18.5
(more )
Specimen
1 N, +/+:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools
