Probe:	Fst cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS8: embryo	Strong	Regionally restricted	7A	Asymmetric expression in the egg cylinder.
TS8: parietal endoderm	Strong	Regionally restricted	7A

Structure	Level	Pattern	Image	Note
TS9: embryo	Strong	Regionally restricted	7B	Asymmetric expression in the egg cylinder.
TS9: parietal endoderm	Strong	Regionally restricted	7B

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong	Regionally restricted	7C	Decidua is strongly positive, except for a narrow zone of decidual cells around the embryo.
TS11: primitive streak	Present		7C
TS11: Reichert's membrane	Strong		7C

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Strong		7D
TS13: future prosencephalon	Absent		7D

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Strong		7E
TS13: future prosencephalon	Absent		7E
TS13: rhombomere 2	Present		7E
TS13: rhombomere 4	Present		7E
TS13: future hindbrain posterior to rhombomere 5	Present	Regionally restricted	7E	Rhombomere 06.

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Strong		7F
TS13: future prosencephalon	Absent		7F

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Strong
TS13: paraxial mesenchyme	Strong
TS13: somite	Strong
TS13: future spinal cord neural plate	Absent

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Regionally restricted	8A	Authors report expression in mesoderm- and ectoderm-derived tissues.
TS20: blood vessel	Present		8A
TS20: diencephalon	Present		8A
TS20: midbrain roof plate	Present		8A
TS20: midbrain	Present	Regionally restricted	8A	A small region in the developing midbrain is negative.
TS20: hindbrain	Weak	Regionally restricted	8A	Floor of rhombencephalon.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted	8B	Authors report expression in mesoderm- and ectoderm-derived tissues.
TS17: diencephalon lateral wall ventricular layer	Present		8B
TS17: midbrain roof plate	Present		8B
TS17: midbrain	Present	Regionally restricted	8B	A small region in the developing midbrain is negative.
TS17: hindbrain	Weak	Regionally restricted	8B	Floor of rhombencephalon.

Structure	Level	Pattern	Image	Note
TS20: midbrain roof plate	Present		9A
TS20: midbrain	Present	Regionally restricted	9A	A small region in the developing midbrain is negative.

Structure	Level	Pattern	Image	Note
TS20: nasal cavity	Present	Regionally restricted	9B	In the roof of the nasal cavity; in particular in the mesenchyme surrounding the olfactory epithelium.
TS20: tongue	Present	Regionally restricted	9B	Mesenchymal structures in the tongue.
TS20: epidermis	Present		9B

Structure	Level	Pattern	Image	Note
TS20: diencephalon lateral wall ventricular layer	Present		9C
TS20: hindbrain	Weak	Regionally restricted	9C	Floor of rhombencephalon.

Structure	Level	Pattern	Image	Note
TS20: cochlear duct epithelium	Present		9D
TS20: cochlear duct mesenchyme	Absent		9D

Structure	Level	Pattern	Image	Note
TS20: stomach mesenchyme	Strong	Regionally restricted	9E	Underlying mesenchymal tissue, but not in the muscle tissue.
TS20: trunk mesenchyme	Present	Regionally restricted	9E	Fascia in the flanks.
TS20: limb	Present	Regionally restricted	9E	Mesenchymal condensations.
TS20: skeleton	Present	Regionally restricted	9E	Mesenchymal condensations in the developing pelvis.

Structure	Level	Pattern	Image	Note
TS20: intestine mesenchyme	Present	Regionally restricted	9F
TS20: axial skeleton	Present	Regionally restricted	9F	Intervertebral disc anlage.

Structure	Level	Pattern	Image	Note
TS20: perioptic mesenchyme	Present	Regionally restricted	9G	Sclera in the developing eye.

Structure	Level	Pattern	Image	Note
TS20: epidermis	Present		9H
TS20: gonad	Present		9H
TS20: heart	Present	Regionally restricted	9H	Sinoauricular septum.

Structure	Level	Pattern	Image	Note
TS21: nasal cavity	Present	Regionally restricted		In the roof of the nasal cavity; in particular in the mesenchyme surrounding the olfactory epithelium.
TS21: cochlear duct epithelium	Present
TS21: vibrissa	Present	Regionally restricted		Expression is restricted to the mesenchyme.
TS21: eye	Present	Regionally restricted		Sclera in the developing eye.
TS21: tooth	Absent
TS21: tongue mesenchyme	Present
TS21: esophagus	Absent
TS21: genital tubercle	Absent
TS21: lung	Absent
TS21: rectum	Absent
TS21: trachea	Absent
TS21: stomach mesenchyme	Present			Underlying mesenchymal tissue, but not in the muscle tissue.
TS21: liver	Absent
TS21: metanephros	Absent
TS21: intestine mesenchyme	Present
TS21: spinal cord	Present	Regionally restricted		Around the lumen of the spinal cord in the tail region.
TS21: brain	Present
TS21: vertebral axis musculature	Present	Regionally restricted		Expression is present in the body wall.
TS21: heart	Present	Regionally restricted		Sinoauricular septum.
TS21: blood vessel	Present			Peripheral blood vessels.
TS21: axial skeleton	Present	Regionally restricted		Expression is present in the intervertebral discs.
TS21: gonad	Present
TS21: pleuro-peritoneal canal	Present			Expression is present in the pleuroperitoneal membrane.
TS21: trunk mesenchyme	Present	Regionally restricted		Expression is present in the fascia in the flanks.
TS21: limb	Present			Mesenchymal condensations.
TS21: shoulder	Present