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Gene Expression Data
Assay Details
Assay
Reference: J:20157 Kopan R, et al., The intracellular domain of mouse Notch: a constitutively activated repressor of myogenesis directed at the basic helix-loop-helix region of MyoD. Development. 1994 Sep;120(9):2385-96
Assay type: RT-PCR
MGI Accession ID: MGI:2135832
Gene symbol: Myf5
Gene name: myogenic factor 5
Probe: Myf5-pE, Myf5-pF
Probe preparation: Double stranded labelled with P32 DNA
Visualized with: Autoradiography
Assay notes: Embryo fragments used for RT-PCR samples contained tissue derived from all germ layers.
Results
Image: 2A Myf5 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
9.5 Day pc, CN + E9.0 (a) TS14: caudal neuropore
TS14: paraxial mesenchyme
Present (b) Not Specified; total RNA CD-1 Not Specified Embryo fragments containing the caudal neuropore (CN) and posterior presomitic mesoderm.
9.5 Day pc, PSM + E9.0 (a) TS14: paraxial mesenchyme Present (b) Not Specified; total RNA CD-1 Not Specified Fragments containing (anterior) presomitic mesoderm (PSM).
9.5 Day pc, 1-4 + E9.0 (a) TS14: somite Present (b) Not Specified; total RNA CD-1 Not Specified Fragments containing the four most posterior somites (1-4).
9.5 Day pc, 5-9 + E9.0 (a) TS14: somite Present (b) Not Specified; total RNA CD-1 Not Specified Fragments containing the next four somites (5-9) anterior to the four most posterior somites.
10 Day pc, CN + E10.0 (a) TS16: caudal neuropore Present (b) Not Specified; total RNA CD-1 Not Specified Embryo fragments containing the caudal neuropore (CN) and posterior presomitic mesoderm.
10 Day pc, PSM + E10.0 (a) TS16: trunk paraxial mesenchyme Present (b) Not Specified; total RNA CD-1 Not Specified Fragments containing (anterior) presomitic mesoderm (PSM).
10 Day pc, 1-4 + E10.0 (a) TS16: trunk somite Present (b) Not Specified; total RNA CD-1 Not Specified Fragments containing the four most posterior somites (1-4).
10 Day pc, 5-9 + E10.0 (a) TS16: trunk somite Present (b) Not Specified; total RNA CD-1 Not Specified Fragments containing the next four somites (5-9) anterior to the four most posterior somites.
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Myf5 expression in both posterior and, to a greater degree, anterior presomitic mesoderm; Myf5 mRNA appears in the caudal neuropore and continues to accumulate during somitogenesis.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/14/2024
MGI 6.23
The Jackson Laboratory