Probe:	pHOX8
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Probe was shortened to approximately 100 bases by alkaline hydrolysis.

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Present	Regionally restricted	4B	Expression in the neural ectoderm of the optic vesicle in a region adjacent to the surface ectoderm.
TS15: olfactory placode	Present	Regionally restricted	4B	Expression in the lateral part of the nasal placode.
TS15: optic cup inner layer	Present		4B
TS15: surface ectoderm	Present	Regionally restricted	4B	Expression in the surface ectoderm near the eye. The expression domain was slightly larger than the optic vesicle.

Structure	Level	Pattern	Image	Note
TS17: eye surface ectoderm	Present
TS17: lens pit	Present
TS17: optic cup inner layer	Present

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Present		4D
TS19: cornea epithelium	Present		4D
TS19: retina	Present	Regionally restricted	4D	Expression in the optic cup in the region to become the neural retina. Transcripts were not distributed uniformly throughout the inner layer of the optic cup, they were absent from the apical portion in the presumptive iris/ciliary body region.

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Absent
TS20: neural retinal epithelium	Absent
TS20: cornea epithelium	Present			Expression in the surface ectoderm of the eye.

Structure	Level	Pattern	Image	Note
TS21: cornea epithelium	Absent