Antibody:	anti-E-cadherin
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	The legend for Figure 1 was provided, but the images were omitted from the text.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted		Authors state that expression is detected in "some regions of the mesencephalon and diencephalon".
TS15: future midbrain	Present	Regionally restricted		Authors state that expression is detected in "some regions of the mesencephalon and diencephalon".

Structure	Level	Pattern	Image	Note
TS18: diencephalon	Present	Regionally restricted		Patchy expression is detected in the posterior end of the mesencephalon and some areas beside the the ventral midline in the mesencephalic flexure, also in parts of the lateral and ventral diencephalon.
TS18: midbrain	Present	Regionally restricted		Patchy expression is detected in the posterior end of the mesencephalon and some areas beside the the ventral midline in the mesencephalic flexure, also in parts of the lateral and ventral diencephalon.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present	Regionally restricted		Patchy expression is detected in the posterior end of the mesencephalon and some areas beside the the ventral midline in the mesencephalic flexure, also in parts of the lateral and ventral diencephalon and the cerebellar rudiments in the metencephalon.
TS20: diencephalon	Present	Regionally restricted		Patchy expression is detected in the posterior end of the mesencephalon and some areas beside the the ventral midline in the mesencephalic flexure, also in parts of the lateral and ventral diencephalon and the cerebellar rudiments in the metencephalon.
TS20: midbrain	Present	Regionally restricted		Patchy expression is detected in the posterior end of the mesencephalon and some areas beside the the ventral midline in the mesencephalic flexure, also in parts of the lateral and ventral diencephalon and the cerebellar rudiments in the metencephalon.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present	Regionally restricted		Patchy expression is detected in cerebellar rudiments in the metencephalon, expression is also increased compared to E12.

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Absent
TS23: brain	Present	Regionally restricted		Expression is detected in the dorsal midline.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted		Expression is detected in the dorsal midline as a double streak which bifurcates in the diencephalon.
TS20: midbrain	Present	Regionally restricted		Expression is detected in the dorsal midline as a double streak which bifurcates in the diencephalon.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present	Regionally restricted		Expression is detected in the posterior end of the mesencephalon and some areas beside the the ventral midline in the mesencephalic flexure, also in parts of the lateral and ventral diencephalon and the cerebellar rudiments in the metencephalon.
TS20: diencephalon	Present	Regionally restricted		Expression is detected in the posterior end of the mesencephalon and some areas beside the the ventral midline in the mesencephalic flexure, also in parts of the lateral and ventral diencephalon and the cerebellar rudiments in the metencephalon.
TS20: midbrain	Present	Regionally restricted		Expression is detected in the posterior end of the mesencephalon and some areas beside the the ventral midline in the mesencephalic flexure, also in parts of the lateral and ventral diencephalon and the cerebellar rudiments in the metencephalon.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present	Regionally restricted		Expression is detected in fibers that grow out of the cerebellar rudiment to the ventral midline.