Probe:	CrabpI cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	1A CRABP	Well-defined labelling in specific regions.
TS11: embryo mesenchyme	Present	Regionally restricted	1A CRABP	Expression throughout primary mesenchyme just lateral to the primitive streak.
TS11: allantois	Present		1A CRABP

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	1B CRABP	Well-defined labelling in specific regions.
TS11: embryo mesenchyme	Present	Regionally restricted	1B CRABP	Expression throughout primary mesenchyme just lateral to the primitive streak.
TS11: primitive streak	Absent		1B CRABP

Structure	Level	Pattern	Image	Note
TS12: head mesenchyme	Present	Regionally restricted	2A CRABP	Transcripts in primary mesenchyme immediately subjacent to epithelium of both midbrain and hindbrain. Cannot distinguish between neural crest and primary mesenchyme in the cranial region.

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	2B CRABP	Transcripts in neural epithelium restricted to a small area of the hindbrain, caudal to the preotic sulcus.
TS12: head mesenchyme	Present	Regionally restricted	2B CRABP	Transcripts in primary mesenchyme immediately subjacent to epithelium of both midbrain and hindbrain. Cannot distinguish between neural crest and primary mesenchyme in the cranial region.

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	3_CRABP	Transcripts in neural epithelium restricted to a small area of the hindbrain, caudal to the preotic sulcus.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain neural ectoderm	Present	Regionally restricted	4A	The region caudal to the preotic sulcus showed the highest level of labelling in the neural epithelium. Labelling extended to the hindbrain region just rostral to preotic sulcus, with the basal region of epithelium most intensely labelled.
TS12: lateral plate mesoderm	Present	Regionally restricted	4A	Transcripts in lateral plate mesoderm dorsal to the heart.
TS12: future hindbrain floor plate	Absent		4A

Structure	Level	Pattern	Image	Note
TS12: future hindbrain neural ectoderm	Present	Regionally restricted	4B
TS12: future hindbrain floor plate	Absent		4B

Structure	Level	Pattern	Image	Note
TS12: embryo mesenchyme	Present	Regionally restricted		Transcripts in caudal primary mesenchyme had diminished.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	4C	Strong expression in specific regions of neural epithelium. Rostral limit at the midbrain-forebrain junction; caudal limit at the level of the first occipital somite.
TS13: future hindbrain	Strong	Regionally restricted	4C	In rostral hindbrain, signal was strongest on basal side of epithelium. More caudally, strong labelling both apically and basally.
TS13: neural tube floor plate	Absent		4C	Midline suprachordal cells (future floor plate of the closed neural tube) were unlabelled.
TS13: head mesenchyme derived from neural crest	Strong	Regionally restricted	4C	High levels of expression in cranial mesenchyme cells in positions corresponding to exprected positions of neural crest cells (e.g. 1st arch).

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	4D	Strong expression in specific regions of neural epithelium. Rostral limit at the midbrain-forebrain junction; caudal limit at the level of the first occipital somite.
TS13: future hindbrain	Strong	Regionally restricted	4D	In rostral hindbrain, signal was strongest on basal side of epithelium. More caudally, strong labelling both apically and basally.
TS13: neural tube floor plate	Absent		4D	Midline suprachordal cells (future floor plate of the closed neural tube) were unlabelled.
TS13: future spinal cord neural fold	Ambiguous		4D	Signal was reduced or absent from the lateral edges of neural epithelium from which crest cells had emigrated.
TS13: head mesenchyme derived from neural crest	Strong	Regionally restricted	4D	High levels of expression in cranial mesenchyme cells in positions corresponding to exprected positions of neural crest cells (e.g. 1st arch).

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	5A CRABP	Strong expression in specific regions of neural epithelium. Rostral limit at the midbrain-forebrain junction; caudal limit at the level of the first occipital somite.
TS13: head mesenchyme derived from neural crest	Strong	Regionally restricted	5A CRABP	High levels of expression in cranial mesenchyme cells in positions corresponding to exprected positions of neural crest cells (e.g. 1st arch).

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	5B CRABP	Strong expression in specific regions of neural epithelium. Rostral limit at the midbrain-forebrain junction; caudal limit at the level of the first occipital somite.
TS13: head mesenchyme derived from neural crest	Strong	Regionally restricted	5B CRABP	High levels of expression in cranial mesenchyme cells in positions corresponding to exprected positions of neural crest cells (e.g. 1st arch).

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	5C CRABP	Strong expression in specific regions of neural epithelium. Rostral limit at the midbrain-forebrain junction; caudal limit at the level of the first occipital somite.
TS13: head mesenchyme derived from neural crest	Strong	Regionally restricted	5C CRABP	High levels of expression in cranial mesenchyme cells in positions corresponding to exprected positions of neural crest cells (e.g. 1st arch).
TS13: trunk mesenchyme	Present	Regionally restricted	5C CRABP	Caudally, strongest signal in mesoderm lateral to the primitive streak, and in presomitic and lateral mesoderm cranial to the primitive streak.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	5D CRABP	Strong expression in specific regions of neural epithelium. Rostral limit at the midbrain-forebrain junction; caudal limit at the level of the first occipital somite.
TS13: head mesenchyme derived from neural crest	Strong	Regionally restricted	5D CRABP	High levels of expression in cranial mesenchyme cells in positions corresponding to exprected positions of neural crest cells (e.g. 1st arch).
TS13: trunk mesenchyme	Present	Regionally restricted	5D CRABP	Caudally, strongest signal in mesoderm lateral to the primitive streak, and in presomitic and lateral mesoderm cranial to the primitive streak.
TS13: lateral plate mesoderm	Present	Regionally restricted	5D CRABP	Diminishing intensity into most recently formed 2 or 3 pairs of somites.
TS13: trunk somite	Present	Regionally restricted	5D CRABP	Diminishing intensity into most recently formed 2 or 3 pairs of somites.
TS13: trunk unsegmented mesenchyme	Present	Regionally restricted	5D CRABP
TS13: mesenchyme derived from head mesoderm	Absent		5D CRABP	Mesoderm-derived cranial mesenchyme appears unlabelled; it is not clear whether labelled subectodermal cells in Fig. 5D originate from neural crest or from mesoderm.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present
TS13: future brain	Present	Regionally restricted		Signal intensity was greater in the hindbrain than in the midbrain.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Absent		6A CRABP
TS14: future hindbrain	Present	Regionally restricted	6A CRABP	Highest signal just rostral to otic placodes; rostral and caudal to this, signal was strongest basally, and a medial unlabelled area was more extensive. A small group of midline supranotochordal cells was unlabelled.
TS14: branchial arch	Present		6A CRABP	Higher levels of transcripts in neural crest-derived mesenchyme of the head and pharyngeal arches than in primary mesenchyme.
TS14: mesenchyme derived from head mesoderm	Present		6A CRABP	Higher levels of transcripts in neural crest-derived mesenchyme of the head and pharyngeal arches than in primary mesenchyme.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	6B CRABP	Highest signal just rostral to otic placodes; rostral and caudal to this, signal was strongest basally, and a medial unlabelled area was more extensive. A small group of midline supranotochordal cells was unlabelled.
TS14: branchial arch	Present		6B CRABP	Higher levels of transcripts in neural crest-derived mesenchyme of the head and pharyngeal arches than in primary mesenchyme.
TS14: mesenchyme derived from head mesoderm	Present		6B CRABP	Higher levels of transcripts in neural crest-derived mesenchyme of the head and pharyngeal arches than in primary mesenchyme.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	6C CRABP	Highest signal just rostral to otic placodes; rostral and caudal to this, signal was strongest basally, and a medial unlabelled area was more extensive. A small group of midline supranotochordal cells was unlabelled.
TS14: head mesenchyme derived from neural crest	Present	Regionally restricted	6C CRABP	Labelling in a group of cells, presumeably neural crest, at the occipital hindbrain level close to the heart, with smaller groups close to the neural tube.
TS14: neural crest	Present	Regionally restricted	6C CRABP	Labelling in a group of cells, presumeably neural crest, at the occipital hindbrain level close to the heart, with smaller groups close to the neural tube.

Structure	Level	Pattern	Image	Note
TS14: head mesenchyme derived from neural crest	Present	Regionally restricted	7A	Labelling in a group of cells, presumeably neural crest, at the occipital hindbrain level close to the heart, with smaller groups close to the neural tube.
TS14: neural crest	Present	Regionally restricted	7A	Labelling in a group of cells, presumeably neural crest, at the occipital hindbrain level close to the heart, with smaller groups close to the neural tube.

Structure	Level	Pattern	Image	Note
TS14: head mesenchyme derived from neural crest	Present	Regionally restricted	7B	Labelling in a group of cells, presumeably neural crest, at the occipital hindbrain level close to the heart, with smaller groups close to the neural tube.
TS14: neural crest	Present	Regionally restricted	7B	Labelling in a group of cells, presumeably neural crest, at the occipital hindbrain level close to the heart, with smaller groups close to the neural tube.

Structure	Level	Pattern	Image	Note
TS14: surface ectoderm	Absent		7C
TS14: trunk somite	Absent		7C

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Present
TS14: mesenchyme derived from lateral plate	Present			Lower signal than at the previous stage.
TS14: embryo	Present	Regionally restricted		Authors state that no labelling was present in endodermal layers.

Structure	Level	Pattern	Image	Note
TS14: embryo	Present	Regionally restricted	8A CRABP	Authors state that the pattern of expression was not very differnt from that seen previously.
TS14: head mesenchyme derived from neural crest	Present		8A CRABP	Higher levels of transcripts in neural crest-derived cells.
TS14: mesenchyme derived from head mesoderm	Present		8A CRABP	Higher levels of transcripts in neural crest-derived cells.
TS14: head mesenchyme	Present	Regionally restricted	8A CRABP	Expression in ring of frontonasal mesenchyme around the forebrain vesicle. Transcripts in pharyngeal arch mesenchyme of the face.
TS14: 1st branchial arch mandibular component mesenchyme	Present		8A CRABP
TS14: prosencephalon	Absent		8A CRABP
TS14: future hindbrain	Present	Regionally restricted	8A CRABP	Particularly strong labelling in the marginal layer.
TS14: trunk mesenchyme	Present	Regionally restricted	8A CRABP	Labelling in mesenchyme associated with the heart, ventral to the dorsal aorta.
TS14: gut	Absent		8A CRABP	No transcripts were found in gut endoderm.
TS14: trunk somite	Absent		8A CRABP

Structure	Level	Pattern	Image	Note
TS14: embryo	Present	Regionally restricted	8B CRABP	Authors state that the pattern of expression was not very differnt from that seen previously.
TS14: mesenchyme derived from head mesoderm	Present		8B CRABP	Higher levels of transcripts in neural crest-derived cells.
TS14: head mesenchyme derived from neural crest	Present		8B CRABP	Higher levels of transcripts in neural crest-derived cells.
TS14: prosencephalon	Absent		8B CRABP
TS14: future midbrain	Present		8B CRABP	Rostral limit of neuroepithelial expression was at the forebrain-midbrain boundary.
TS14: otic pit	Present		8B CRABP
TS14: gut	Absent		8B CRABP	No transcripts were found in gut endoderm.
TS14: trunk somite	Absent		8B CRABP

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted		Labelling extended to the most rostral levels of the trunk neural tube.
TS14: central nervous system	Present			High levels of transcripts in cranial ganglia V, VII/VIII, IX and X.
TS14: mesenchyme derived from lateral plate	Present
TS14: limb	Present	Regionally restricted		Transcripts in the mesoderm of the limbs.