Probe:	RAR-beta-cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	1A RAR-beta	Well-defined labelling in specific regions. Lower expression in midline than in lateral regions.
TS11: embryo mesenchyme	Present	Regionally restricted	1A RAR-beta	Expression is not clearly tissue-related, except in the most rostral area; present in mesenchyme but not in overlying neural epithelium.
TS11: neural ectoderm	Present	Regionally restricted	1A RAR-beta	Expression is not clearly tissue-related, except in the most rostral area; present in mesenchyme but not in overlying neural epithelium.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	1B RAR-beta	Well-defined labelling in specific regions. Lower expression in midline than in lateral regions.

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Absent		2A RAR-beta
TS12: foregut diverticulum endoderm	Present	Regionally restricted	2A RAR-beta	Transcripts were also found in foregut endoderm.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain neural ectoderm	Present	Regionally restricted	2B RAR-beta	Transcripts within neural epithelium restricted to caudal hindbrain levels.

Structure	Level	Pattern	Image	Note
TS12: embryo mesenchyme	Present	Regionally restricted		Authors state that labelling in the mesoderm was regionally restricted.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	5A RAR-beta	Expressed from just rostral to the first somite, extending caudally as far as the limit of neural tube closure.

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present		5B RAR-beta
TS13: neural ectoderm	Present	Regionally restricted	5B RAR-beta	Expressed from just rostral to the first somite, extending caudally as far as the limit of neural tube closure.

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present		5C RAR-beta
TS13: sinus venosus	Present		5C RAR-beta
TS13: neural ectoderm	Present		5C RAR-beta	Expressed from just rostral to the first somite, extending caudally as far as the limit of neural tube closure.

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present		5D RAR-beta
TS13: sinus venosus	Present		5D RAR-beta
TS13: neural ectoderm	Present	Regionally restricted	5D RAR-beta	Expressed from just rostral to the first somite, extending caudally as far as the limit of neural tube closure.
TS13: future spinal cord neural fold	Absent		5D RAR-beta

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present
TS13: head somite	Absent
TS13: trunk somite	Absent

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	6A RAR-beta	Trancripts in caudal hindbrain, extending caudally into the trunk neural tube as far as the caudal neuropore.
TS14: neural tube	Present	Regionally restricted	6A RAR-beta	Trancripts in caudal hindbrain, extending caudally into the trunk neural tube as far as the caudal neuropore.
TS14: head mesenchyme	Present	Regionally restricted	6A RAR-beta	Transcripts detected in frontonasal mesenchyme.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	6B RAR-beta	Trancripts in caudal hindbrain, extending caudally into the trunk neural tube as far as the caudal neuropore.
TS14: neural tube	Present	Regionally restricted	6B RAR-beta	Trancripts in caudal hindbrain, extending caudally into the trunk neural tube as far as the caudal neuropore.
TS14: future spinal cord neural fold	Absent		6B RAR-beta	Not labelled in the open neural folds.
TS14: gut	Present	Regionally restricted	6B RAR-beta	Transcripts detected in gut endoderm.
TS14: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	6B RAR-beta	Transcripts extended into the medial part of the ectoderm-derived epithelial covering of the mandibular arch.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	6C RAR-beta	Trancripts in caudal hindbrain, extending caudally into the trunk neural tube as far as the caudal neuropore.
TS14: neural tube	Present	Regionally restricted	6C RAR-beta	Trancripts in caudal hindbrain, extending caudally into the trunk neural tube as far as the caudal neuropore.
TS14: mesenchyme derived from splanchnopleure	Present	Regionally restricted	6C RAR-beta	Transcripts present in splanchnopleuric mesoderm of the upper trunk.
TS14: trunk somite	Absent		6C RAR-beta

Structure	Level	Pattern	Image	Note
TS14: embryo	Present	Regionally restricted	8A RAR-beta	Authors state that the pattern of expression was not very different from that seen previously.
TS14: head mesenchyme derived from neural crest	Present		8A RAR-beta	Higher levels of transcripts in neural crest-derived cells.
TS14: mesenchyme derived from head mesoderm	Present		8A RAR-beta	Higher levels of transcripts in neural crest-derived cells.
TS14: head mesenchyme	Present	Regionally restricted	8A RAR-beta	Expression in a ring of frontonasal mesenchyme around the forebrain vesicle.
TS14: future hindbrain	Present	Regionally restricted	8A RAR-beta	Rostral limit in hindbrain neuroepithelium corresponds to the rhombomere 6/7 boundary, just caudal to otic vesicles.
TS14: neural tube	Present	Regionally restricted	8A RAR-beta	Expression extends along the whole length of the closed region of the neural tube.
TS14: 1st branchial arch mandibular component mesenchyme	Absent		8A RAR-beta
TS14: gut	Present	Regionally restricted	8A RAR-beta	Transcripts were found in gut endoderm.
TS14: trunk somite	Absent		8A RAR-beta

Structure	Level	Pattern	Image	Note
TS14: otic pit epithelium	Absent
TS14: intermediate mesenchyme	Present
TS14: mesenchyme derived from lateral plate	Present
TS14: limb	Present	Regionally restricted		Transcripts in the most proximal mesenchyme of the developing limbs.