Probe:	DW3-le
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		1A	No transcripts were detected in sections of several embryos.

Structure	Level	Pattern	Image	Note
TS12: future spinal cord neural plate	Strong	Regionally restricted	1B	Expression was strong in the caudal region.
TS12: neural tube	Strong	Regionally restricted	1B	Expression was strong in the caudal region, extending up to the rostral part of the future spinal cord.
TS12: trunk unsegmented mesenchyme	Present		1B	Expression in presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS12: future spinal cord neural plate	Strong	Regionally restricted	1C	Expression was strong in the caudal region.
TS12: neural tube	Strong	Regionally restricted	1C	Expression was strong in the caudal region, extending up to the rostral part of the future spinal cord.
TS12: embryo ectoderm	Present	Regionally restricted	1C	Expression in the rostral neural tube, in the headfold region, did not exceed background levels.
TS12: head somite	Strong		1C
TS12: trunk somite	Strong		1C
TS12: head mesenchyme	Present	Regionally restricted	1C	Absence of labelling in the head mesenchyme contrasted with the intense staining in the somites.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	4B , 4A	Expression was detected throughout the entire caudal tube in the germinative neuroepithelium.
TS15: future brain	Present	Regionally restricted	4A , 4B	Transcripts appear rostrally in the primitive brain on neurons of the synencephalon and of the mesencephalic and motor nuclei of the trigeminal nerve.

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Regionally restricted		Expression was detected in the earliest-born neurons in the rostral spinal cord, the motor neurons in the incipient ventral horn.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	4C	There was intense, uniform labelling of the caudal neural tube.
TS17: brain	Present	Regionally restricted	4C	Expression was detected in postmitotic neurons.
TS17: future spinal cord	Present	Regionally restricted	4C	Expression was detected in postmitotic neurons in the cervical spinal cord. The signal did not exceed background levels in the germinal epithelium, while expression was strong in the developing ventral horn containing the differentiating motor neurons.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	4E , 4D	Caudally, strong expression in proliferating neuroepithelium. Weak staining at lumbar levels, and virtually undetectable further rostrally. Expression in differentiating motor neurons in cervical region is first visible at thoracic levels.
TS17: neural tube	Present	Regionally restricted	4D , 4E	There was intense, uniform labelling of the caudal neural tube.

Structure	Level	Pattern	Image	Note
TS14: heart	Present
TS14: organ system	Present	Regionally restricted		Expression in a variety other tissues, such as spleen.
TS14: trunk somite	Strong	Regionally restricted		Intense expression in somites, particularly in the forming myotome.

Structure	Level	Pattern	Image	Note
TS19: nervous system	Present	Regionally restricted	5A	Expression was detected in differentiating neurons along the entire neuraxis but was low or absent in the dividing cells of the ventricular layer and the inner parts of the intermediate layer.
TS19: neural tube	Present	Regionally restricted	5A	Caudally, ventral parts of the neural tube are sparsely populated by postmitotic neurons; the germinal layer is negative. Rostrally, lateral parts of the tube are strongly positive.
TS19: brain	Strong	Regionally restricted	5A	Postmitotic neurons in all brain vesicles are heavily labelled.
TS19: olfactory epithelium	Present		5A	Expression was seen in the olfactory epithelium and in the olfactory roots.
TS19: cranial ganglion	Present		5A
TS19: dorsal root ganglion	Present		5A

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Strong	Regionally restricted	5B	Expression was intense in the ventral parts of the spinal cord containing the differentiating motor neurons.
TS19: brain	Present	Regionally restricted	5B	Expression was detected in all brain vesicles in postmitotic neurones, except in the mesencephalic-metencephalic isthmic region.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	5C	Postmitotic neurones are stained over the length of the neural tube.
TS20: brain	Present	Regionally restricted	5C	Expression is interrupted on the basal plate at the mesencephalic-metencephalic border.