Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Flt1tm1Msh
Name:	FMS-like tyrosine kinase 1; targeted mutation 1, Masabumi Shibuya
MGI ID:	MGI:1934260
Synonyms:	Flt-1TK-, flt tk-, VEGFR1-DeltaTKD
Gene:	Flt1  Location: Chr5:147498414-147662821 bp, - strand  Genetic Position: Chr5, 87.01 cM, cytoband G
Alliance:	Flt1tm1Msh page

Germline Transmission:	Earliest citation of germline transmission: J:49507
Parent Cell Line:	J1 (ES Cell)
Strain of Origin:	129S4/SvJae

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A PGK-neomycin resistance cassette replaced exon 17, which encodes the amino terminal region of the tyrosine kinase domain. Northern blot analysis of homozygous mutant embryos using a 5' cDNA probe detected a ~3kb mRNA, and detected no 7kb full length transcript using a 3' probe. Immunoblot analysis of lung tissue from homozygous mutant mice detected the endogenous soluble form of the protein, and a novel truncated form that lacks the cytoplasmic region. No full length protein was expressed from this allele. (J:49507)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Flt1 Mutation:	73 strains or lines available

Original:	J:49507 Hiratsuka S, et al., Flt-1 lacking the tyrosine kinase domain is sufficient for normal development and angiogenesis in mice. Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9349-54
All:	38 reference(s)