Probe:	Hox-5.1 probe1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Regionally restricted	1D
TS20: spinal cord	Present		1D	Trancripts were most concentrated in dorsal parts of the spinal cord.

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present		2C
TS20: medulla oblongata	Present	Regionally restricted	2C	The anterior boundary was found in the mid-myelencephalon.

Structure	Level	Pattern	Image	Note
TS20: C1 vertebral cartilage condensation	Weak		3C/D	Weak labeling especially in ventral parts.
TS20: C2 vertebral cartilage condensation	Strong		3C/D
TS20: cervical vertebral cartilage condensation	Present	Graded	3C/D
TS20: thoracic vertebral cartilage condensation	Present	Graded	3C/D	Labeling gradually decreased to background levels by prevertebra 14 (T7).
TS20: lumbar vertebral cartilage condensation	Present	Graded	3C/D	Labeling intensity increased from prevertebrae 20 - 25.
TS20: sacral vertebral cartilage condensation	Present	Graded	3C/D	Labeling declined from prevertebrae 27 - 30.
TS20: sacral vertebral pre-cartilage condensation	Present	Graded	3C/D	Labeling declined from prevertebrae 27 - 30.
TS20: lung	Absent		3C/D

Structure	Level	Pattern	Image	Note
TS20: thymus primordium	Present		5D
TS20: thyroid gland	Present		5D
TS20: pharynx	Present	Regionally restricted	5D	Expression in the floor of the pharynx posterior and anterior to the thyroid duct but absent in posterior parts of the pharyngeal floor.
TS20: trachea	Absent		5D

Structure	Level	Pattern	Image	Note
TS20: mesonephros	Present		6D
TS20: metanephros	Present		6D
TS20: liver	Absent		6D
TS20: stomach	Present	Regionally restricted	6D	Transcripts in the stomach were restricted to patches located in the peripheral zone of the wall and are absent from mesodermal components.
TS20: testis	Strong	Regionally restricted	6D	Transcripts were located mainly within the peripheral mesenchymal tissue of the testis and were less intense or absent within the testis cords.

Structure	Level	Pattern	Image	Note
TS20: gut	Present	Regionally restricted		Expression in posterior regions of the intestine.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present	Regionally restricted	7D	Transcripts were confined to more posterior parts, lateral plate mesoderm, somtatic mesoderm, and neurectoderm.
TS13: neural ectoderm	Present	Regionally restricted	7D	Transcripts were confined to more posterior parts, lateral plate mesoderm, somtatic mesoderm, and neurectoderm.
TS13: trunk paraxial mesenchyme	Present	Regionally restricted	7D	Transcripts were confined to more posterior parts, lateral plate mesoderm, somtatic mesoderm, and neurectoderm.

Structure	Level	Pattern	Image	Note
TS15: trunk mesenchyme	Present	Regionally restricted	8D	Expression in lateral plate mesoderm as it contacted and surrounded the gut endoderm.
TS15: future spinal cord	Present		8D	Expression in somatic mesoderm and neurectoderm.
TS15: trunk paraxial mesenchyme	Present		8D	Expression in somatic mesoderm and neurectoderm.

Structure	Level	Pattern	Image	Note
TS17: lung	Weak	Regionally restricted	9E	Weak expression in the mesodermal components of the lung which and absence of expression in the adjacent lining endothelium.

Structure	Level	Pattern	Image	Note
TS17: stomach	Present	Regionally restricted	9F	Uniform distribution over the mesodermal components of the stomach wall.
TS17: stomach epithelium	Absent		9F	Absence of expression in the lining endothelium.