Gene Expression Data
Assay Details
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Assay
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Reference:
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J:21617
Gunther T, et al., Open brain, a new mouse mutant with severe neural tube defects, shows altered gene expression patterns in the developing spinal cord. Development. 1994 Nov;120(11):3119-30
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1933408
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Results
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Specimen
3G:
embryonic day 12.5
(more  )
Specimen
3G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: basal columns |
Present |
Single cells |
3G
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Isl1 expression was used as a marker for differentiation motor neurons.
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Specimen
3H:
embryonic day 12.5; Rab23opb/ Rab23opb
(more )
Note: The original opb carriers were of a mixed outbred NMRI background. The opb mutant embryos used in this study were derived subsequent to at least 6 crosses of opb mutant males to wild-type C57Bl/6 females.
Specimen
3H:
(close )
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Genetic Background:
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involves: C57BL/6 * NMRI
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Mutant Allele(s):
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Rab23opb/Rab23opb
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The original opb carriers were of a mixed outbred NMRI background. The opb mutant embryos used in this study were derived subsequent to at least 6 crosses of opb mutant males to wild-type C57Bl/6 females.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: basal columns |
Present |
Single cells |
3H
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The number of Isl1-expressing motor neuron precursor cells was greatly reduced in the mutant embryos.
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Analysis Tools