Gene Expression Data
Assay Details
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Assay
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Reference:
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J:68215
Xue Y, et al., Mesodermal patterning defect in mice lacking the Ste20 NCK interacting kinase (NIK). Development. 2001 May;128(9):1559-72
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1933099
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Gene symbol:
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En1
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Gene name:
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engrailed 1
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Probe:
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En1 probe4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6 E:
embryonic day 8.5
(more  )
Specimen
6 E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future hindbrain |
Present |
Regionally restricted |
6E
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Expression at the midbrain/hindbrain boundary.
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| TS13: future midbrain |
Present |
Regionally restricted |
6E
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Expression at the midbrain/hindbrain boundary.
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Specimen
6 F:
(close )
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Genetic Background:
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either: (involves: 129S/SvEv * Swiss Webster) or (involves: CD-1 * Swiss Webster)
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Mutant Allele(s):
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Map4k4tm1Eys/Map4k4tm1Eys
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future hindbrain |
Present |
Regionally restricted |
6F
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Same relative distribution and levels as wild-type.
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| TS13: future midbrain |
Present |
Regionally restricted |
6F
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Same relative distribution and levels as wild-type.
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Analysis Tools
