Antibody:	anti-CRABP I
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Kreisler -/- mutants at dpc 9.5 have an absence of morphological segmentation posterior to the boundary between r3 and r4. There are two Crabp1 antibodies. Either antibody may have been used in this assay. See MGI:4460229 for details about the second antibody.

Structure	Level	Pattern	Image	Note
TS15: rhombomere 4	Strong		3A
TS15: rhombomere 5	Strong		3A
TS15: rhombomere 6	Strong		3A
TS15: rhombomere 7	Present		3A	Expression is fading out.
TS15: rhombomere 2	Weak		3A

Structure	Level	Pattern	Image	Note
TS15: rhombomere 4	Strong		3B	Strong expression in a region what normally would be r4 and no expression posterior to this region.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present		3C	Expression in neural crest cells which migrate as two discrete streams anterior and posterior to the otocyst.
TS15: facio-acoustic preganglion complex	Present		3C	Expression was in neural crest cells in the developing facial and glossopharyngeal ganglia.
TS15: glossopharyngeal-vagus preganglion complex	Present		3C	Expression was in neural crest cells in the developing facial and glossopharyngeal ganglia.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present		3D	Expression in a single continuous group of neural crest cells adjacent to the hindbrain that diverge into two streams entering the second and third branchial arches.