Gene Expression Data
Assay Details
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Assay
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Reference:
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J:19631
McKay IJ, et al., The kreisler mouse: a hindbrain segmentation mutant that lacks two rhombomeres. Development. 1994 Aug;120(8):2199-211
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1932338
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Gene symbol:
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Egr2
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Gene name:
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early growth response 2
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Probe:
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Krox20 probe14
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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Kreisler -/- mutants at dpc 9.5 have an absence of morphological segmentation posterior to the boundary between r3 and r4.
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Results
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Specimen
2A:
embryonic day 9.5
(more  )
Note: Approximately 22 somites.
Specimen
2A:
(close  )
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Genetic Background:
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involves: C3H/He * CBA
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Approximately 22 somites.
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Specimen
2B:
embryonic day 9.5; Mafbkr/ Mafbkr
(more )
Note: Approximately 22 somites.
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Mafbkr/Mafbkr
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Approximately 22 somites.
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Specimen
2C:
embryonic day 9.5; Mafbkr/ Mafbkr
(more )
Note: Approximately 22 somites. More dorsal section of the same embryo as in B.
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Mafbkr/Mafbkr
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Approximately 22 somites. More dorsal section of the same embryo as in B.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: rhombomere 3 |
Present |
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2C
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This domain of expression persists in kreisler embryos (up to 25 somites) when wild-type embryos have ceased to show expression in r3. This is a more dorsal section of same embryo as in B.
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Specimen
Table 1/not shown:
embryonic day 9.0
(more )
Note: 17 and 19 somites.
Specimen
Table 1/not shown:
(close )
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Genetic Background:
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involves: C3H/He * CBA
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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17 and 19 somites.
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Specimen
Table 1/not shown:
embryonic day 9.5
(more )
Note: 21, 22, 24 and 25 somites.
Specimen
Table 1/not shown:
(close )
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Genetic Background:
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involves: C3H/He * CBA
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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21, 22, 24 and 25 somites.
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Specimen
Table 1/not shown:
embryonic day 9.0; Mafbkr/ Mafbkr
(more )
Note: 18 and 19 somites.
Specimen
Table 1/not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Mafbkr/Mafbkr
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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18 and 19 somites.
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Specimen
Table 1/not shown:
embryonic day 9.5; Mafbkr/ Mafbkr
(more )
Note: 22-25 somites.
Specimen
Table 1/not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Mafbkr/Mafbkr
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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22-25 somites.
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Specimen
not shown:
embryonic day 8.0
(more )
Note: 3-5 somites.
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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3-5 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future hindbrain |
Present |
Regionally restricted |
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A single domain of expression corresponding to future r3 in 3-5 somite embryos.
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Specimen
not shown:
embryonic day 8.0; Mafbkr/ Mafbkr
(more )
Note: 3-5 somites.
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Mafbkr/Mafbkr
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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3-5 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future hindbrain |
Present |
Regionally restricted |
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A single domain of expression corresponding to future r3 in 3-5 somite embryos.
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Specimen
5A:
embryonic day 8.0; Mafbkr/Mafb +
(more )
Note: 7 somites.
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Mafbkr/Mafb+
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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7 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future hindbrain |
Present |
Regionally restricted |
5A
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Expression in a pattern corresponding to r3 and r5.
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Specimen
5B:
embryonic day 8.0; Mafbkr/ Mafbkr
(more )
Note: 7 somites.
Specimen
5B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Mafbkr/Mafbkr
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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7 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future hindbrain |
Present |
Regionally restricted |
5B
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Expression in a domain corresponding to r3 and absence of expression in an area corresponding to r5.
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Analysis Tools