Gene Expression Data
Assay Details
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Assay
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Reference:
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J:38388
Dupe V, et al., In vivo functional analysis of the Hoxa-1 3' retinoic acid response element (3'RARE). Development. 1997 Jan;124(2):399-410
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1932120
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Gene symbol:
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Egr2
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Gene name:
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early growth response 2
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Probe:
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Krox-20 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4A,4C:
embryonic day 8.75
(more  )
Note: 9-12 somites.
Specimen
4A,4C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.75
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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9-12 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: anterior pro-rhombomere |
Present |
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4A
,
4C
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Expression in pre-r3 and pre-r5 exhibited sharp boundaries, with expression levels in pre-r3 lower than in pre-r5.
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| TS12: posterior pro-rhombomere |
Present |
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4A
,
4C
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Expression in pre-r3 and pre-r5 exhibited sharp boundaries, with expression levels in pre-r3 lower than in pre-r5.
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Specimen
not shown:
embryonic day 8.75; Hoxa1tm2Ipc/Hoxa1 +
(more )
Note: 9-12 somites.
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * SJL
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Mutant Allele(s):
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Hoxa1tm2Ipc/Hoxa1+
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Age:
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embryonic day 8.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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9-12 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: rhombomere 3 |
Present |
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Expression in pre-r3 and pre-r5 exhibited sharp boundaries, with expression levels in pre-r3 lower than in pre-r5.
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| TS13: rhombomere 5 |
Present |
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Expression in pre-r3 and pre-r5 exhibited sharp boundaries, with expression levels in pre-r3 lower than in pre-r5.
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Specimen
4B,4D:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * SJL
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Mutant Allele(s):
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Hoxa1tm2Ipc/Hoxa1tm2Ipc
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Age:
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embryonic day 8.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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9-12 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: rhombomere 3 |
Present |
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4B
,
4D
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In 5 out of 15 mutant embryos, boundaries and size of pre-r3 and pre-r5 expression domains were altered, with an enlarged but weaker pre-r3 domain and reduced pre-r5 domain.
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| TS13: rhombomere 5 |
Present |
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4B
,
4D
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In 5 out of 15 mutant embryos, boundaries and size of pre-r3 and pre-r5 expression domains were altered, with an enlarged but weaker pre-r3 domain and reduced pre-r5 domain.
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Specimen
not shown:
embryonic day 8.0; Hoxa1tm2Ipc/ Hoxa1tm2Ipc
(more )
Note: At a slightly earlier stage than 8.75 dpc; 7-8 somites.
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * SJL
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Mutant Allele(s):
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Hoxa1tm2Ipc/Hoxa1tm2Ipc
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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At a slightly earlier stage than 8.75 dpc; 7-8 somites.
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Analysis Tools