Gene Expression Data
Assay Details
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Assay
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Reference:
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J:3103
Geada AM, et al., Sequence and embryonic expression of the murine Hox-3.5 gene. Development. 1992 Oct;116(2):497-506
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1932063
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Gene symbol:
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Hoxc4
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Gene name:
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homeobox C4
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Probe:
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cDNA 4-1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
5C:
embryonic day 12.5
(more  )
Specimen
5C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Regionally restricted |
5C
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The anterior Hoxc4 boundary was located anterior to that of the anterior Hoxc5 boundary.
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Specimen
6A:
embryonic day 12.5
(more )
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
6A/B
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Within the prevertebral column, no labeling was detected in pv 1-3, but low levels of transcripts were seen in ventral parts of pv 4. Transcripts increased in abundance over pv 5-7, were most abundant over pv 7-13/14, and then declined more posteriorly.
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| TS20: esophagus |
Present |
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6A/B
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Transcripts were abundant in the oesophagus, with only very low levels in the trachea and lung.
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| TS20: lung |
Present |
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6A/B
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Transcripts were abundant in the oesophagus, with only very low levels in the trachea and lung.
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| TS20: trachea |
Present |
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6A/B
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Transcripts were abundant in the oesophagus, with only very low levels in the trachea and lung.
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Specimen
7B,7D:
embryonic day 10.5
(more )
Specimen
7B,7D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
7A/B
,
7C/D
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The anterior Hoxc4 boundary did not coincide with a morphologically visible rhombomere junction, but was located posterior to anterior Hoxb4 boundary (at the junction of rhombomeres 6 and 7) by about one rhombomere length.
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Specimen
8A:
embryonic day 9.5
(more )
Specimen
8A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Regionally restricted |
8A/B
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The anterior Hoxc4 boundary did not coincide with a morphologically visible rhombomere junction, but was located posterior to anterior Hoxb4 boundary (at the junction of rhombomeres 6 and 7) by about one rhombomere length.
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Specimen
9B:
embryonic day 12.5
(more )
Specimen
9B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
9A/B
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Transcripts were most abundant ventrally and centrally within the mantle layer.
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| TS20: spinal cord mantle layer |
Present |
Regionally restricted |
9A/B
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Transcripts were most abundant ventrally and centrally within the mantle layer.
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Specimen
10B:
embryonic day 12.5
(more )
Specimen
10B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Male
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metanephros |
Present |
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10A/B
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Transcripts were detected within the metanephric kidney.
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| TS20: testis |
Absent |
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10A/B
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Transcripts were not detected within the testis.
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Analysis Tools