Gene Expression Data
Assay Details
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Assay
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Reference:
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J:62912
MacPhee DJ, et al., Differential involvement of Na(+),K(+)-ATPase isozymes in preimplantation development of the mouse. Dev Biol. 2000 Jun 15;222(2):486-98
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1931978
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Gene symbol:
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Atp1a1
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Gene name:
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ATPase, Na+/K+ transporting, alpha 1 polypeptide
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Antibody:
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mAb 6H
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
4. A.:
embryonic day 2.0
(more  )
Note: 4-cell embryo. Embryos were fixed in a series of methanol/PBS dilutions.
Specimen
4. A.:
(close  )
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Genetic Background:
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CF-1
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Age:
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embryonic day 2.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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4-cell embryo. Embryos were fixed in a series of methanol/PBS dilutions.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-cell stage embryo |
Present |
Regionally restricted |
4A
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Expression is detected in the blastomere membranes that were in contact or close apposition to one another.
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Specimen
4. B.:
embryonic day 2.5
(more )
Note: 8-cell embryo. Embryos were fixed in a series of methanol/PBS dilutions.
Specimen
4. B.:
(close )
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Genetic Background:
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CF-1
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Age:
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embryonic day 2.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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8-cell embryo. Embryos were fixed in a series of methanol/PBS dilutions.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Present |
Regionally restricted |
4B
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Expression is detected throughout the blastomere membranes.
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Specimen
4. C.:
embryonic day 3.0
(more )
Note: Embryo was at the Morula stage. Embryos were fixed in a series of methanol/PBS dilutions.
Specimen
4. C.:
(close )
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Genetic Background:
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CF-1
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Age:
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embryonic day 3.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Embryo was at the Morula stage. Embryos were fixed in a series of methanol/PBS dilutions.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Strong |
Regionally restricted |
4C
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Expression is detected in the blastomere membranes.
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Specimen
4. D.:
embryonic day 3.5
(more )
Note: Embryo was at the blastocyst stage. Embryos were fixed in a series of methanol/PBS dilutions.
Specimen
4. D.:
(close )
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Genetic Background:
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CF-1
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Embryo was at the blastocyst stage. Embryos were fixed in a series of methanol/PBS dilutions.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: inner cell mass |
Present |
Regionally restricted |
4D
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Expression is detected in the basolateral membranes of the mural and polar trophectoderm and the membranes of the inner cell mass however little or no expression is detected in apical membranes of the mural and polar trophectoderm.
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| TS4: mural trophectoderm |
Present |
Regionally restricted |
4D
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Expression is detected in the basolateral membranes of the mural and polar trophectoderm and the membranes of the inner cell mass however little or no expression is detected in apical membranes of the mural and polar trophectoderm.
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| TS4: polar trophectoderm |
Present |
Regionally restricted |
4D
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Expression is detected in the basolateral membranes of the mural and polar trophectoderm and the membranes of the inner cell mass however little or no expression is detected in apical membranes of the mural and polar trophectoderm.
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