Gene Expression Data
Assay Details
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Assay
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Reference:
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J:63681
Martinez-Barbera JP, et al., The homeobox gene Hesx1 is required in the anterior neural ectoderm for normal forebrain formation. Dev Biol. 2000 Jul 15;223(2):422-30
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1931456
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Gene symbol:
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Six3
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Gene name:
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sine oculis-related homeobox 3
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Probe:
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Six3 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6A:
embryonic day 7.5
(more  )
Note: Neural plate stage.
Specimen
6A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Neural plate stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: neural ectoderm |
Present |
Regionally restricted |
6A
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Expression in anterior neural ectoderm.
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Specimen
6B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Hesx1tm1Icar/Hesx1tm1Icar
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Neural plate stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: neural ectoderm |
Present |
Regionally restricted |
6B
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Expression in anterior neural ectoderm.
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Specimen
6C:
embryonic day 7.5
(more )
Note: Late headfold stage.
Specimen
6C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Late headfold stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: neural ectoderm |
Present |
Regionally restricted |
6C
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Expression in anterior neural ectoderm.
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Specimen
6D:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Hesx1tm1Icar/Hesx1tm1Icar
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Late headfold stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: neural ectoderm |
Present |
Regionally restricted |
6D
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Expression in anterior neural ectoderm.
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Specimen
6E:
embryonic day 8.0
(more )
Note: 3-5 somites.
Specimen
6E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-5 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
Regionally restricted |
6E
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Expression in forebrain, most rostral neuroectoderm. Expression in the medial aspect of the anterior neural plate.
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Specimen
6F:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Hesx1tm1Icar/Hesx1tm1Icar
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-5 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
Regionally restricted |
6F
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The expression domain (forebrain, most rostral neuroectoderm) is smaller than in wild-type littermates. Expression in the medial apect of the anterior neural plate was significantly reduced in mutant.
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