Gene Expression Data
Assay Details
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Assay
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Reference:
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J:55583
Tribioli C, et al., The murine Bapx1 homeobox gene plays a critical role in embryonic development of the axial skeleton and spleen. Development. 1999 Dec;126(24):5699-711
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1930220
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Gene symbol:
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Nkx3-2
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Gene name:
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NK3 homeobox 2
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Probe:
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Bapx1 probe1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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This probe detects a portion of the Bapx1 mRNA that is present in the null allele. This probe marks null cells that normally would express Bapx1 proteins.
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Results
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Specimen
6 S:
embryonic day 12.5
(more  )
Specimen
6 S:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: axial skeleton |
Present |
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6S
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Similar transcript distribution among the cells of the prevertebrae in the wild-type and null embryos is observed (see assay note above).
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Specimen
6 T:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J)
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Mutant Allele(s):
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Nkx3-2tm1.1Tlu/Nkx3-2tm1.1Tlu
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: axial skeleton |
Present |
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6T
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Similar transcript distribution among the cells of the prevertebrae in the wild-type and null embryos is observed (see assay note above).
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Analysis Tools