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Gene Expression Data
Assay Details
Assay
Reference: J:66592 Liu A, et al., EN and GBX2 play essential roles downstream of FGF8 in patterning the mouse mid/hindbrain region. Development. 2001 Jan;128(2):181-91
Assay type: RNA in situ
MGI Accession ID: MGI:1929097
Gene symbol: Pax5
Gene name: paired box 5
Probe: 5sg8
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 2. A: embryonic day 9.5; En1tm1Alj/En1+, En2tm1Alj/En2tm1Alj (more )
Structure Level Pattern Image Note
TS15: embryo Present 2A
Specimen 2. A': embryonic day 9.5; En1tm2Alj/En1tm2Alj, En2tm2Alj/En2tm2Alj (more )
Structure Level Pattern Image Note
TS15: embryo Absent 2A'
Specimen 3. C: embryonic day 8.0; En1tm1Alj/En1+, En2tm1Alj/En2tm1Alj (more )
Note: Embryo is at the 7 somite stage.
Structure Level Pattern Image Note
TS12: future hindbrain Present Regionally restricted 3C Expression is detected in the mesencephalon and metencephalon.
TS12: future midbrain Present Regionally restricted 3C Expression is detected in the mesencephalon and metencephalon.
Specimen 3. C': embryonic day 8.0; En1tm2Alj/En1tm2Alj, En2tm2Alj/En2tm2Alj (more )
Note: Embryo is at the 7 somite stage.
Structure Level Pattern Image Note
TS12: future hindbrain Present Regionally restricted 3C' Expression is detected in the mesencephalon and metencephalon but is less than in wild type and more regionally restricted.
TS12: future midbrain Present Regionally restricted 3C' Expression is detected in the mesencephalon and metencephalon but is less than in wild type and more regionally restricted.
Specimen 3. H: embryonic day 8.5; En1tm1Alj/En1+, En2tm1Alj/En2tm1Alj (more )
Note: Embryo is at the 11 somite stage.
Structure Level Pattern Image Note
TS13: future hindbrain Present Regionally restricted 3H Expression is detected in the mesencephalon and metencephalon.
TS13: future midbrain Present Regionally restricted 3H Expression is detected in the mesencephalon and metencephalon.
Specimen 3. H': embryonic day 8.5; En1tm2Alj/En1tm2Alj, En2tm2Alj/En2tm2Alj (more )
Note: Embryo is at the 11 somite stage.
Structure Level Pattern Image Note
TS13: future hindbrain Weak Regionally restricted 3H' Expression is detected in the mesencephalon and metencephalon.
TS13: future midbrain Weak Regionally restricted 3H' Expression is detected in the mesencephalon and metencephalon.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory