Probe:	Hoxb2 probe5
Probe preparation:	labelled with S35
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	1A	hybridization in the neural tube ending in rhombomere 3 and in the VII/VIII cranial ganglion complex.
TS14: facio-acoustic neural crest	Present		1A	hybridization in the neural tube ending in rhombomere 3 and in the VII/VIII cranial ganglion complex.
TS14: rhombomere 3	Present		1A	hybridization in the neural tube ending in rhombomere 3 and in the VII/VIII cranial ganglion complex.

Structure	Level	Pattern	Image	Note
TS14: 2nd branchial arch	Present	Regionally restricted	1B	Expression in the center of the second branchial arch and in a more posterior domain, as well as within the neural tube itself.
TS14: neural tube	Present		1B

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch	Absent		1C , 1C'
TS14: 2nd branchial arch	Present		1C , 1C'	Arch 2 and more posterior regions express Hoxb2 largely due to expression in the neural crest.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch	Absent		1D' , 1D	No expression on the surface of arch 1, but expression is present in adjacent posterior ectodermal tissue.
TS14: surface ectoderm	Present	Regionally restricted	1D' , 1D	No expression on the surface of arch 1, but expression is present in adjacent posterior ectodermal tissue.

Structure	Level	Pattern	Image	Note
TS15: 2nd branchial arch mesenchyme derived from neural crest	Present		2A

Structure	Level	Pattern	Image	Note
TS15: 2nd branchial arch ectoderm	Present	Regionally restricted	2C , 2B	Expression is seen on the surface of the second arch.
TS15: 1st branchial arch	Absent		2B , 2C

Structure	Level	Pattern	Image	Note
TS15: 2nd branchial arch	Present		2D	In the reconstructed embryo expression can be seen to be within all areas lateral of the hindbrain colonized by the neural crest and extending ventrally into the second arch.
TS15: 2nd branchial arch mesenchyme derived from neural crest	Present		2D	In the reconstructed embryo expression can be seen to be within all areas lateral of the hindbrain colonized by the neural crest and extending ventrally into the second arch.
TS15: future hindbrain	Present		2D	In the reconstructed embryo expression can be seen to be within all areas lateral of the hindbrain colonized by the neural crest and extending ventrally into the second arch.
TS15: 1st branchial arch	Absent		2D

Structure	Level	Pattern	Image	Note
TS13: rhombomere 2	Absent		3A	The neural plate is labeled, and only on one side, this section is at the level of anaterior cutoff of expression between rhombomere 2 (left side not expressing) and rhombomere 3 (right side expressing). More anterior sections are negative.
TS13: future hindbrain	Present	Regionally restricted	3A	The neural plate is labeled, and only on one side, this section is at the level of anterior cutoff of expression between rhombomere 2 (left side not expressing) and rhombomere 3 (right side expressing).
TS13: rhombomere 3	Present	Regionally restricted	3A	The neural plate is labeled, and only on one side, this section is at the level of anterior cutoff of expression between rhombomere 2 (left side not expressing) and rhombomere 3 (right side expressing).
TS13: surface ectoderm	Absent		3A	No labeling of surface ectoderm although it is continuous with the neural plate.
TS13: extraembryonic component	Strong		3A	Extra-embryonic membranes showed intense labeling.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present		3B	A more posterior section shows labeling of both sides of the neural plate with no expression in the other embryonic parts of the section.
TS13: rhombomere 3	Present	Regionally restricted	3B
TS13: surface ectoderm	Absent		3B	No labeling of surface ectoderm although it is continuous with the neural plate.
TS13: extraembryonic component	Strong		3B	Adjacent extra-embryonic membranes showed intense labeling.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present		3C	A patch of expression lateral to the dorsal edge of the neural plate, this site of labeling is in neural crest and is continuous with the neural plate extending ventrolaterally first on one side and then on both sides.
TS13: rhombomere 4	Present	Regionally restricted	3C
TS13: surface ectoderm	Absent		3C	No labeling of surface ectoderm although it is continuous with the neural plate.
TS13: extraembryonic component	Strong		3C	Adjacent extra-embryonic membranes showed intense labeling.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present		3D	A region on the right of the section where only neural plate is labeled.
TS13: rhombomere 5	Present	Regionally restricted	3D
TS13: surface ectoderm	Absent		3D	No labeling of surface ectoderm although it is continuous with the neural plate.
TS13: extraembryonic component	Strong		3D	Adjacent extra-embryonic membranes showed intense labeling.