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Gene Expression Data
Assay Details
Assay
Reference: J:65599 Gaufo GO, et al., Hoxb1 controls effectors of sonic hedgehog and mash1 signaling pathways. Development. 2000 Dec;127(24):5343-54
Assay type: Immunohistochemistry
MGI Accession ID: MGI:1928093
Gene symbol: Nkx2-2
Gene name: NK2 homeobox 2
Antibody: 74.5A5
Detection system: Secondary antibody coupled to Texas Red
Results
Specimen 4a: embryonic day 11.5 (more )
Note: Double labeled: red - Nkx2-2; green - Hoxb1 (assay MGI:1928087). Theiler stage 18 was chosen to remain consistent with other annotations from this manuscript.
Structure Level Pattern Image Note
TS18: rhombomere 4 floor plate Present Regionally restricted 4a Expression is in the inner layer of the ventral neuroepithelium juxtaposed to the floorplate.
Specimen 4b: embryonic day 11.5; Hoxb1tm5Mrc/Hoxb1tm5Mrc (more )
Note: Theiler stage 18 was chosen to remain consistent with other annotations from this manuscript.
Structure Level Pattern Image Note
TS18: rhombomere 4 mantle layer Present 4b
TS18: rhombomere 4 Present Regionally restricted 4b Dorsal expression is reduced compared to wild-type embryos.
Specimen 6g: embryonic day 11.5 (more )
Note: Double labeled: red - Nkx2-2; green - Phox2b (assay MGI:1928136). Theiler stage 18 was chosen to remain consistent with other annotations from this manuscript.
Structure Level Pattern Image Note
TS18: rhombomere 4 Present Regionally restricted 6g Expression is in the ventral progenitor domain.
Specimen 6h: embryonic day 11.5; Hoxb1tm5Mrc/Hoxb1tm5Mrc (more )
Note: Double labeled: red - Nkx2-2; green - Phox2b (assay MGI:1928136). Theiler stage 18 was chosen to remain consistent with other annotations from this manuscript.
Structure Level Pattern Image Note
TS18: rhombomere 4 marginal layer Present Regionally restricted 6h
TS18: rhombomere 4 ventricular layer Present Regionally restricted 6h
TS18: rhombomere 4 Present Regionally restricted 6h The expression in the ventricular proliferation domain is absent, although expression is present elsewhere in the rhombomere.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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