Gene Expression Data
Assay Details
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Assay
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Reference:
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J:32071
Lyons GE, et al., alpha-Actin proteins and gene transcripts are colocalized in embryonic mouse muscle. Development. 1991;111(2):451-4
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1927890
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Gene symbol:
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Actc1
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Gene name:
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actin, alpha, cardiac muscle 1
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Antibody:
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anti-alpha-actin
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Detection system:
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Secondary antibody coupled to alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 9.25
(more  )
Note: 18-somite.
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.25
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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18-somite.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: heart |
Present |
Regionally restricted |
1A
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Alpha-actin proteins are expressed in cardiac myocytes.
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| TS14: trunk dermomyotome |
Weak |
Regionally restricted |
1A
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Protein levels in the myotomes of somites at 9.25 dpc are very low but detectable at the level of the cervical somites, but absent in more caudal somites.
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| TS14: gut |
Absent |
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1A
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The smooth muscle in the primitve gut between the neural tube and the heart does not stain positively with the antibody.
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Specimen
1C:
embryonic day 11.5
(more )
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: trunk dermomyotome |
Strong |
Uniform |
1C
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Uniform alpha-actin protein expression throughout the myotome.
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Specimen
1E:
embryonic day 10.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: tail dermomyotome |
Strong |
Regionally restricted |
1E
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Proteins are expressed at high levels in rostral myotomes.
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Specimen
1G:
embryonic day 13.0
(more )
Specimen
1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hindlimb |
Present |
Regionally restricted |
1G
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Proteins are expressed in the muscle groups.
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Specimen
not shown:
embryonic day 8.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Present |
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The levels of alpha-actin proteins at dpc 8.0 are comparable to that seen in Fig. 1A, B.
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Specimen
not shown:
embryonic day 11.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
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Alpha-actin protein is also detected in limb buds.
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| TS19: hindlimb bud |
Present |
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Alpha-actin protein is also detected in limb buds.
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Analysis Tools