Gene Expression Data
Assay Details
Assay
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Reference:
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J:65299
Dubreuil V, et al., The phox2b transcription factor coordinately regulates neuronal cell cycle exit and identity. Development. 2000 Dec;127(23):5191-201
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1926870
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Gene symbol:
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Hes5
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Gene name:
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hes family bHLH transcription factor 5
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Probe:
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Hes5 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1G:
embryonic day 10.5; Phox2btm1Jbr/Phox2b +
(more )
Specimen
1G:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2b+
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS17: rhombomere 4 ventricular layer |
Strong |
Patchy |
1G
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Expression was not uniform. Clusters of cells with high expression levels juxtaposed cells where expression was barely detectable.
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Specimen
1H:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2btm1Jbr
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS17: rhombomere 4 ventricular layer |
Present |
Patchy |
1H
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Expression was not uniform. Expression was reduced in some regions compared to the heterozygous specimen.
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