Lefty2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:60975 Chang H, et al., Smad5 is essential for left-right asymmetry in mice. Dev Biol. 2000 Mar 1;219(1):71-8
Assay type:	RNA in situ
MGI Accession ID:	MGI:1891686
Gene symbol:	Lefty2
Gene name:	left-right determination factor 2

Probe:

Results

2E ©

Specimen 2E: embryonic day 8.0 (more

)
Note: 1-2 somites. frontal view

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Strong	Regionally restricted	2E	Expression is detected on the left side of the lateral plate mesoderm.
TS12: future hindbrain floor plate	Weak	Regionally restricted	2E	Expression is detected on the left side of the putative floor plate.
TS12: future midbrain floor plate	Weak	Regionally restricted	2E	Expression is detected on the left side of the putative floor plate.
TS12: future prosencephalon floor plate	Weak	Regionally restricted	2E	Expression is detected on the left side of the putative floor plate.
TS12: neural tube floor plate	Weak	Regionally restricted	2E	Expression is detected on the left side of the putative floor plate.

2F ©

Specimen 2F: embryonic day 8.0 (more

)
Note: 1-2 somites. lateral view

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Strong	Regionally restricted	2F	Expression is detected on the left side of the lateral plate mesoderm.
TS12: future hindbrain floor plate	Weak	Regionally restricted	2F	Expression is detected on the left side of the putative floor plate.
TS12: future midbrain floor plate	Weak	Regionally restricted	2F	Expression is detected on the left side of the putative floor plate.
TS12: future prosencephalon floor plate	Weak	Regionally restricted	2F	Expression is detected on the left side of the putative floor plate.
TS12: neural tube floor plate	Weak	Regionally restricted	2F	Expression is detected on the left side of the putative floor plate.

2G ©

Specimen 2G: embryonic day 8.0; Smad5^tm1Zuk/Smad5^tm1Zuk (more

)
Note: 1-2 somites. frontal view

Structure	Level	Image
TS12: future hindbrain floor plate	Absent	2G
TS12: future midbrain floor plate	Absent	2G
TS12: future prosencephalon floor plate	Absent	2G
TS12: neural tube floor plate	Absent	2G
TS12: lateral plate mesoderm	Weak	2G

2H ©

Specimen 2H: embryonic day 8.0; Smad5^tm1Zuk/Smad5^tm1Zuk (more

)
Note: 1-2 somites. lateral view

Structure	Level	Image
TS12: future hindbrain floor plate	Absent	2H
TS12: future midbrain floor plate	Absent	2H
TS12: future prosencephalon floor plate	Absent	2H
TS12: neural tube floor plate	Absent	2H
TS12: lateral plate mesoderm	Weak	2H

Specimen Table 1: embryonic day 8.0; Smad5^tm1Zuk/Smad5^tm1Zuk (more

)
Note: 3-4 somites. Data is from multiple specimens listed in table 1.

Structure	Level	Note
TS12: lateral plate mesoderm	Present	Expression of Leftb was bilateral in 7 of 9 mutant embryos.
TS12: future hindbrain floor plate	Absent	Expression was absent in 9 of 9 embryos.
TS12: future midbrain floor plate	Absent	Expression was absent in 9 of 9 embryos.
TS12: future prosencephalon floor plate	Absent	Expression was absent in 9 of 9 embryos.
TS12: neural tube floor plate	Absent	Expression was absent in 9 of 9 embryos.

Specimen Table 1: embryonic day 8.0; Smad5^tm1Zuk/Smad5^tm1Zuk (more

)
Note: 3-4 somites. Data is from multiple specimens listed in table 1.

Structure	Level	Note
TS12: future hindbrain floor plate	Absent	Expression was absent in 9 of 9 embryos.
TS12: future midbrain floor plate	Absent	Expression was absent in 9 of 9 embryos.
TS12: future prosencephalon floor plate	Absent	Expression was absent in 9 of 9 embryos.
TS12: neural tube floor plate	Absent	Expression was absent in 9 of 9 embryos.
TS12: lateral plate mesoderm	Absent	Expression was absent in 2 of 9 embryos.

Specimen Table 1: embryonic day 8.0; Smad5^tm1Zuk/Smad5^tm1Zuk (more

)
Note: 5-6 somites. Data is from multiple specimens listed in table 1.

Structure	Level	Note
TS12: lateral plate mesoderm	Present	Expression of Leftb was bilateral in 3 of 3 mutant embryos.
TS12: future hindbrain floor plate	Absent	Expression was absent in 3 of 3 cases.
TS12: future midbrain floor plate	Absent	Expression was absent in 3 of 3 cases.
TS12: future prosencephalon floor plate	Absent	Expression was absent in 3 of 3 cases.
TS12: neural tube floor plate	Absent	Expression was absent in 3 of 3 cases.

Specimen Table 1: embryonic day 8.5; Smad5^tm1Zuk/Smad5^tm1Zuk (more

)
Note: 7-8 somites. Data is from multiple specimens listed in table 1.

Structure	Level	Note
TS13: lateral plate mesoderm	Present	Expression of Leftb was bilateral in 2 of 3 mutant embryos.
TS13: future hindbrain floor plate	Absent	Expression was absent in 3 of 3 cases.
TS13: future midbrain floor plate	Absent	Expression was absent in 3 of 3 cases.
TS13: future prosencephalon floor plate	Absent	Expression was absent in 3 of 3 cases.
TS13: neural tube floor plate	Absent	Expression was absent in 3 of 3 cases.

Specimen Table 1: embryonic day 8.5; Smad5^tm1Zuk/Smad5^tm1Zuk (more

)
Note: 7-8 somites. Data is from multiple specimens listed in table 1.

Structure	Level	Note
TS13: future hindbrain floor plate	Absent	Expression was absent in 3 of 3 cases.
TS13: future midbrain floor plate	Absent	Expression was absent in 3 of 3 cases.
TS13: future prosencephalon floor plate	Absent	Expression was absent in 3 of 3 cases.
TS13: neural tube floor plate	Absent	Expression was absent in 3 of 3 cases.
TS13: lateral plate mesoderm	Absent	Expression was absent in 1 of 3 cases.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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