Gene Expression Data
Assay Details
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Assay
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Reference:
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J:63213
Drewell RA, et al., Deletion of a silencer element disrupts H19 imprinting independently of a DNA methylation epigenetic switch. Development. 2000 Aug;127(16):3419-28
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1889530
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Gene symbol:
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H19
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Gene name:
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H19, imprinted maternally expressed transcript
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Probe:
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H19 cDNA4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3a +/+:
embryonic day 13.5
(more  )
Specimen
3a +/+:
(close  )
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Genetic Background:
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involves: 129 * BALB/c
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3b H19Silk/+:
embryonic day 13.5; Rr128tm1Masu/Rr128 +
(more )
Note: The mutant allele was maternally inherited in this animal.
Specimen
3b H19/+:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/c
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Mutant Allele(s):
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Rr128tm1Masu/Rr128+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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The mutant allele was maternally inherited in this animal.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: embryo |
Present |
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3b
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
3c H19delta3/+:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
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Mutant Allele(s):
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Rr128tm1Masu/Rr128+
H19tm1Lda/H19+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: liver |
Present |
Single cells |
3c
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Specimen
3d +/H19Silk:
embryonic day 13.5; Rr128tm1Masu/Rr128 +
(more )
Note: The mutant allele was paternally inherited in this animal.
Specimen
3d +/H19:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/c
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Mutant Allele(s):
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Rr128tm1Masu/Rr128+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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The mutant allele was paternally inherited in this animal.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: embryo |
Present |
Regionally restricted |
3d
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Expression is in a subset of tissues when compared to wild-type embryos.
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Specimen
4a:
embryonic day 13.5; Rr128tm1Masu/Rr128 +
(more )
Note: The mutant allele was maternally inherited in this animal.
Specimen
4a:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/c
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Mutant Allele(s):
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Rr128tm1Masu/Rr128+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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The mutant allele was maternally inherited in this animal.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: gut |
Present |
Regionally restricted |
4a
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Expression is in the gastric epithelium and smooth muscle cells.
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Specimen
4b:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
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Mutant Allele(s):
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Rr128tm1Masu/Rr128+
H19tm1Lda/H19+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: gut |
Strong |
Regionally restricted |
4b
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Expression is only in epithelial cells.
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Specimen
4c:
embryonic day 13.5; Rr128tm1Masu/Rr128 +
(more )
Note: The mutant allele was maternally inherited in this animal.
Specimen
4c:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/c
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Mutant Allele(s):
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Rr128tm1Masu/Rr128+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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The mutant allele was maternally inherited in this animal.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: stomach |
Present |
Regionally restricted |
4c
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There is expression in the epithelium and the smooth muscle.
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Specimen
4d:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
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Mutant Allele(s):
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Rr128tm1Masu/Rr128+
H19tm1Lda/H19+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: stomach |
Present |
Regionally restricted |
4d
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There is expression in the epithelium and no expression in the smooth muscle.
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Specimen
4e:
embryonic day 13.5; Rr128tm1Masu/Rr128 +
(more )
Note: The mutant allele was maternally inherited in this animal.
Specimen
4e:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/c
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Mutant Allele(s):
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Rr128tm1Masu/Rr128+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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The mutant allele was maternally inherited in this animal.
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Specimen
4f:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
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Mutant Allele(s):
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Rr128tm1Masu/Rr128+
H19tm1Lda/H19+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4g:
embryonic day 13.5; Rr128tm1Masu/Rr128 +
(more )
Note: The mutant allele was maternally inherited in this animal.
Specimen
4g:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/c
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Mutant Allele(s):
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Rr128tm1Masu/Rr128+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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The mutant allele was maternally inherited in this animal.
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Specimen
4h:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
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Mutant Allele(s):
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Rr128tm1Masu/Rr128+
H19tm1Lda/H19+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
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Mutant Allele(s):
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Rr128tm1Masu/Rr128+
H19tm1Lda/H19+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools