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Gene Expression Data
Assay Details
Assay
Reference: J:60095 Barrow JR, et al., Roles of Hoxa1 and Hoxa2 in patterning the early hindbrain of the mouse. Development. 2000 Mar;127(5):933-44
Assay type: RNA in situ
MGI Accession ID: MGI:1353413
Gene symbol: Epha4
Gene name: Eph receptor A4
Probe: Epha4 probe6
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 5E: embryonic day 9.5; Hoxa1tm2Mrc/Hoxa1+, Hoxa2tm1Mrc/Hoxa2+ (more )
Structure Level Pattern Image Note
TS15: rhombomere 3 Strong 5E
TS15: rhombomere 5 Strong 5E
TS15: rhombomere 2 Moderate 5E
TS15: rhombomere 4 Moderate 5E
TS15: rhombomere 6 Moderate 5E
Specimen 5F: embryonic day 9.5; Hoxa1tm2Mrc/Hoxa1+, Hoxa2tm1Mrc/Hoxa2tm1Mrc (more )
Structure Level Pattern Image Note
TS15: rhombomere 3 Strong Regionally restricted 5F There is a reduced rhombomere 3 expression domain and the anterior limit of expression is ill-defined.
TS15: rhombomere 2 Moderate 5F
TS15: rhombomere 4 Moderate 5F
TS15: rhombomere 6 Moderate 5F
TS15: rhombomere 5 Strong 5F
Specimen 5G: embryonic day 9.5; Hoxa1tm1Mrc/Hoxa1tm1Mrc (more )
Structure Level Pattern Image Note
TS15: rhombomere 3 Present Regionally restricted 5G There is a slightly larger expression domain than in the heterozygotes.
TS15: rhombomere 5 Absent 5G The rhombomere 5 band is missing in this embryo; rhombomere 5 markers were never expressed in this mutant.
Specimen 5H: embryonic day 9.5; Hoxa1tm2Mrc/Hoxa1tm2Mrc, Hoxa2tm1Mrc/Hoxa2tm1Mrc (more )
Structure Level Pattern Image Note
TS15: rhombomere 2 Present Regionally restricted 5H Expression extends to the midpoint of rhombomere 2.
TS15: rhombomere 3 Present Regionally restricted 5H The rhombomere 3 domain is severely reduced.
TS15: rhombomere 5 Absent 5H
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory