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Gene Expression Data
Assay Details
Assay
Reference: J:60095 Barrow JR, et al., Roles of Hoxa1 and Hoxa2 in patterning the early hindbrain of the mouse. Development. 2000 Mar;127(5):933-44
Assay type: RNA in situ
MGI Accession ID: MGI:1353412
Gene symbol: Hoxb2
Gene name: homeobox B2
Probe: Hoxb2 probe4
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 5A: embryonic day 9.5; Hoxa1tm2Mrc/Hoxa1+, Hoxa2tm1Mrc/Hoxa2+ (more )
Structure Level Pattern Image Note
TS15: rhombomere 3 Strong 5A
TS15: rhombomere 4 Strong 5A
TS15: rhombomere 5 Strong 5A
TS15: head mesenchyme derived from neural crest Present Regionally restricted 5A Expression is in the neural crest emanating from rhombomeres 4 and 6.
TS15: rhombomere 6 Moderate 5A
Specimen 5B: embryonic day 9.5; Hoxa1tm2Mrc/Hoxa1+, Hoxa2tm1Mrc/Hoxa2tm1Mrc (more )
Structure Level Pattern Image Note
TS15: rhombomere 3 Strong 5B The rhombomere 2/3 boundary is ill-defined and rhombomere 3 is reduced in size.
TS15: rhombomere 4 Strong 5B The rhombomere 2/3 boundary is ill-defined and rhombomere 3 is reduced in size.
TS15: rhombomere 5 Strong 5B The rhombomere 2/3 boundary is ill-defined and rhombomere 3 is reduced in size.
TS15: rhombomere 6 Moderate 5B
TS15: head mesenchyme derived from neural crest Present Regionally restricted 5B Expression is in the neural crest emanating from rhombomeres 4 and 6.
Specimen 5C: embryonic day 9.5; Hoxa1tm1Mrc/Hoxa1tm1Mrc (more )
Structure Level Pattern Image Note
TS15: rhombomere 3 Present 5C
TS15: rhombomere 4 Strong Regionally restricted 5C The authors term this the r4/6 region. In this mutant it is believed that rhombomere 5 is missing.
TS15: rhombomere 6 Strong Regionally restricted 5C The authors term this the r4/6 region. In this mutant it is believed that rhombomere 5 is missing.
TS15: head mesenchyme derived from neural crest Absent 5C There appears to be no neural crest emanating from the r4/6 segment.
Specimen 5D: embryonic day 9.5; Hoxa1tm2Mrc/Hoxa1tm2Mrc, Hoxa2tm1Mrc/Hoxa2tm1Mrc (more )
Structure Level Pattern Image Note
TS15: rhombomere 2 Present Regionally restricted 5D In the double mutant the anterior limit of expression extended to the midpoint of rhombomere 2.
TS15: rhombomere 3 Present Regionally restricted 5D Rhombomere 3 appears severely reduced in size.
TS15: rhombomere 4 Present Regionally restricted 5D The authors term this the r4/6 region. In this mutant it is believed that rhombomere 5 is missing.
TS15: rhombomere 6 Present Regionally restricted 5D The authors term this the r4/6 region. In this mutant it is believed that rhombomere 5 is missing.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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