Antibody:	anti krox-20
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	1A	There is a narrow band of cells (3-4 cell widths) in presumptive rhombomere 3.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	1B	Expression extends anteriorly to the level of the pre-otic sulcus.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	1C	Expression extends anteriorly to the level of the pre-otic sulcus.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	1D	Expression was shifted caudally to the level of rhombomere 4 with expression in only a few cells in rhombomere 3.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 3	Strong		3A
TS13: rhombomere 5	Strong		3A
TS13: neural ectoderm	Present	Regionally restricted	3A	Expression is in dorsal cells posterior to rhombomere 5. These may be presumptive neural crest cells.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 3	Present		3B	Expression is not significantly altered in this mutant.
TS13: rhombomere 5	Present		3B	Expression is not significantly altered in this mutant.
TS13: future hindbrain posterior to rhombomere 5 neural crest	Present	Regionally restricted	3B	Expression is not significantly altered in this mutant. Expression is in dorsal cells posterior to rhombomere 5. These may be presumptive neural crest cells.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 3	Present	Patchy	3C
TS13: rhombomere 4	Present	Patchy	3C
TS13: rhombomere 5	Present	Regionally restricted	3C	Only scattered cells at the level of rhombomere 5 had expression.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 4	Present	Patchy	3D
TS13: rhombomere 3	Present	Regionally restricted	3D	Very few expressing cells were detected at the level of rhombomere 3.
TS13: rhombomere 5	Present	Regionally restricted	3D	Only scattered cells at the level of rhombomere 5 had expression.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 3	Present	Regionally restricted	3E	Expression has expanded more fully and is less patchy than at earlier stages.
TS13: neural ectoderm	Present	Regionally restricted	3E	Expression is in the dorsal neuroepithelium at the level of caudal rhombomere 5 and rhombomere 6.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 3	Present	Patchy	3F	Expression has expanded, although somewhat weakly into the rhombomere 3 domain relative to double mutants at earlier stages.
TS13: rhombomere 4	Present		3F
TS13: rhombomere 5	Present		3F