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Gene Expression Data
Assay Details
Assay
Reference: J:56581 Daubas P, et al., Myf5 is a novel early axonal marker in the mouse brain and is subjected to post-transcriptional regulation in neurons. Development. 2000 Jan;127(2):319-31
Assay type: RT-PCR
MGI Accession ID: MGI:1352723
Gene symbol: Myf5
Gene name: myogenic factor 5
Probe: Myf5-p1, Myf5-p3
Probe preparation: Double stranded labelled with P32 DNA
Visualized with: Autoradiography
Assay notes: The primers used in this assay span exons 1 -3. RT-PCR products were revealed by hybrdization with radiolabeled internal oligonucleotides.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 1500 bp (a) 390 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
1 Treated sample: no data stored
2 E11.5 TS19: tail Present Present 1 µg; total RNA Not Specified Not Specified
3 Lane not used in this assay
4 E11.5 TS19: midbrain Present Present 1 µg; total RNA Not Specified Not Specified ventral mesencephalon p1 region
Notes:
(a) The 1500 bp fragment is only seen if the blot is overexposed.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory