About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:59281 Manaia A, et al., Lmo2 and GATA-3 associated expression in intraembryonic hemogenic sites. Development. 2000 Feb;127(3):643-53
Assay type: RNA in situ
MGI Accession ID: MGI:1352416
Gene symbol: Gata3
Gene name: GATA binding protein 3
Probe: Gata3 cDNA3
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1E top panel: embryonic day 8.0 (more )
Note: Late head-fold stage.
Structure Level Pattern Image Note
TS12: gut Present 1E top
TS12: embryo ectoderm Present Regionally restricted 1E top Expression is in the lateral ectoderm.
TS12: allantois Present Regionally restricted 1E top Expression is where hematopoietic cluster-bearing vessels develop.
TS12: splanchnopleure Present Regionally restricted 1E top Expression is where hematopoietic cluster-bearing vessels develop.
Specimen 1E bottom panel: embryonic day 8.0 (more )
Note: Late head-fold stage, 3 somites.
Structure Level Pattern Image Note
TS12: allantois Present 1E bottom
TS12: splanchnopleure Present 1E bottom
TS12: blood island Absent 1E bottom
Specimen 1I: embryonic day 9.5-10.0 (more )
Note: 25-30 somites.
Structure Level Pattern Image Note
TS14: 2nd branchial arch Present 1I Authors state expression is detected in the 2nd, 3rd and 4th Branchial Arches; the aortic region and the Mesonephros.
TS14: 3rd branchial arch Present 1I Authors state expression is detected in the 2nd, 3rd and 4th Branchial Arches; the aortic region and the Mesonephros.
TS14: dorsal aorta Present 1I Authors state expression is detected in the 2nd, 3rd and 4th Branchial Arches; the aortic region and the Mesonephros.
TS14: pronephros Present 1I Authors state expression is detected in the 2nd, 3rd and 4th Branchial Arches; the aortic region and the Mesonephros.
TS14: embryo mesenchyme Present Regionally restricted 1I Expression is in the posterior ventral mesoderm.
Specimen 2E: embryonic day 8.0 (more )
Note: 5-somite stage. Ventral view.
Structure Level Pattern Image Note
TS12: splanchnopleure Present Regionally restricted 2E Expression is caudal.
TS12: allantois Present Regionally restricted 2E Expression is at the basis of the allantois.
Specimen 2F,G: embryonic day 8.0 (more )
Note: 5-somite stage. The section in (F) is truncal and in (G) is caudal.
Structure Level Pattern Image Note
TS12: embryo endoderm Present Regionally restricted 2F , 2G Expression is in the area underlying the aorta.
TS12: embryo ectoderm Present Regionally restricted 2G , 2F Expression is in the lateral ectoderm.
Specimen 3A: embryonic day 10.0-10.5 (more )
Note: This section is from the aortic region.
Structure Level Pattern Image Note
TS16: trunk mesenchyme Present Regionally restricted 3A Expression is in mesodermal cells underlying the aorta. A few cells display a stronger signal.
Specimen 3B: embryonic day 11.0-11.5 (more )
Note: This section is from the aortic region.
Structure Level Pattern Image Note
TS19: dorsal aorta Present Regionally restricted 3B Expression is at the basis of an aortic cluster.
TS19: trunk mesenchyme Present Regionally restricted 3B Expression is within mesodermal patches beneath the aorta.
Specimen 3D: embryonic day 11.0-11.5 (more )
Note: This section is from the aortic region.
Structure Level Pattern Image Note
TS19: dorsal aorta Present Patchy 3D
Specimen 3E: embryonic day 11.0-11.5 (more )
Note: Double labeled: yellow - Gata3; blue - Th (assay MGI:1352435); red - overlap.
Structure Level Pattern Image Note
TS19: dorsal aorta Present Patchy 3E
Specimen 3F: embryonic day 11.0-11.5 (more )
Structure Level Pattern Image Note
TS19: dorsal aorta Present Patchy 3F
Specimen 3G: embryonic day 11.0-11.5 (more )
Structure Level Pattern Image Note
TS19: dorsal aorta Present Patchy 3G
Specimen 3H: embryonic day 11.0-11.5 (more )
Note: Double labeled: yellow - Gata3; blue - Ptprc (assay MGI:1352429); red - overlap.
Structure Level Pattern Image Note
TS19: dorsal aorta Present Patchy 3H
Specimen 4B: embryonic day 10.0 (more )
Structure Level Pattern Image Note
TS16: hepatic primordium Present Uniform 4B This is before hematopoietic colinization.
Specimen 4C: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: liver Strong Spotted 4C
Specimen 4G: embryonic day 10.0 (more )
Note: 30-35 somites.
Structure Level Pattern Image Note
TS16: optic cup Present 4G Expression is in the optic and otic placodes.
TS16: otocyst Present 4G Expression is in the optic and otic placodes.
TS16: 1st branchial arch Present 4G The thymic rudiment is clearly labeled.
TS16: 2nd branchial arch Present 4G The thymic rudiment is clearly labeled.
TS16: 3rd branchial arch Present 4G The thymic rudiment is clearly labeled.
TS16: 4th branchial arch Present 4G The thymic rudiment is clearly labeled.
Specimen 4H top: embryonic day 10.0 (more )
Note: 30-35 somites. Sagittal section
Structure Level Pattern Image Note
TS16: 1st branchial arch mesenchyme Present Regionally restricted 4H top Expression is in the mesoderm.
TS16: 2nd branchial arch mesenchyme Present 4H top Expression is in the mesoderm.
TS16: 3rd branchial arch mesenchyme Present 4H top Expression is in the mesoderm.
TS16: 4th branchial arch mesenchyme Present 4H top Expression is in the mesoderm.
TS16: 3rd branchial pouch endoderm Present Regionally restricted 4H top Expression is restricted to the thymic epithelium.
Specimen 4H bottom: embryonic day 10.0 (more )
Note: 30-35 somites.
Structure Level Pattern Image Note
TS16: 3rd branchial pouch endoderm Present Regionally restricted 4H bottom Expression is on the side of the thymic rudiment opposite the foregut.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory