Gene Expression Data
Assay Details
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Assay
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Reference:
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J:58081
Lettice L, et al., The dominant hemimelia mutation uncouples epithelial-mesenchymal interactions and disrupts anterior mesenchyme formation in mouse hindlimbs. Development. 1999 Nov;126(21):4729-36
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1347777
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Gene symbol:
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Alx4
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Gene name:
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aristaless-like homeobox 4
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Probe:
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Alx4 probe4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
4D +/+:
embryonic day 10.5
(more  )
Specimen
4D +/+:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4E Dh/+:
embryonic day 10.5; Dh/Dh +
(more )
Specimen
4E Dh/+:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Dh/Dh+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud mesenchyme |
Strong |
Regionally restricted |
4E
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Expression is reduced in size and the distal limit appears more proximal in this mutant.
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Specimen
4F Dh/Dh:
embryonic day 10.5; Dh/ Dh
(more )
Specimen
4F Dh/Dh:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Dh/Dh
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud mesenchyme |
Present |
Regionally restricted |
4F
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In mutants, the anterior expression is lacking and a small domain of proximal expression remains.
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Analysis Tools