Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:58081
Lettice L, et al., The dominant hemimelia mutation uncouples epithelial-mesenchymal interactions and disrupts anterior mesenchyme formation in mouse hindlimbs. Development. 1999 Nov;126(21):4729-36
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1347776
|
|
Gene symbol:
|
Msx1
|
|
Gene name:
|
msh homeobox 1
|
|
Probe:
|
Msx1 probe7
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
4A +/+:
embryonic day 10.5
(more  )
Specimen
4A +/+:
(close  )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud mesenchyme |
Strong |
Regionally restricted |
4A
|
Expression is strong at the anterior margin and is lower in the posterior and distal margin.
|
Specimen
4B Dh/+:
embryonic day 10.5; Dh/Dh +
(more )
Specimen
4B Dh/+:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Dh/Dh+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud mesenchyme |
Strong |
Regionally restricted |
4B
|
The anterior domain of expression is smaller in this mutant.
|
Specimen
4C Dh/Dh:
embryonic day 10.5; Dh/ Dh
(more )
Specimen
4C Dh/Dh:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Dh/Dh
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud mesenchyme |
Present |
Regionally restricted |
4C
|
The anterior domain of expression is missing in mutant embryos. The posterior and distal domains are unaffected.
|
|
Analysis Tools
