Probe:	pS3H7
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: amnion ectoderm	Present		2C
TS11: chorion ectoderm	Absent		2C
TS11: embryo ectoderm	Present	Regionally restricted	2C	Expression is in the inner embryonic ectoderm.
TS11: extraembryonic visceral endoderm	Absent		2C

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Present		3B
TS13: surface ectoderm	Present	Regionally restricted	3B	Expression is posterior.
TS13: hindgut diverticulum	Present	Regionally restricted	3B	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS17: nervous system	Absent		4B
TS17: embryo mesenchyme	Strong	Regionally restricted	4B	Expression is in interstitial mesenchyme.
TS17: liver	Present		4B
TS17: outflow tract	Strong		4B
TS17: extraembryonic vascular system	Present	Regionally restricted	4B	Expression surrounds the umbilical vessels.
TS17: primitive ventricle	Present	Regionally restricted	4B	Expression is in the ventricular wall.

Structure	Level	Pattern	Image	Note
TS17: embryo mesenchyme	Present	Regionally restricted	5B	Expression is between the dorsal aorta and the spinal chord.

Structure	Level	Pattern	Image	Note
TS19: 4th ventricle	Weak	Regionally restricted	5D	Expression is at the junction of the spinal chord central canal and the fourth ventrical.
TS19: future spinal cord	Weak	Regionally restricted	5D	Expression is at the junction of the spinal chord central canal and the fourth ventrical.
TS19: brain	Present	Regionally restricted	5D	Expression is in the loose mesenchyme and endothelium of the pia matter.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	5F	Expression at the midbrain/hindbrain junction is in the pia matter and in small patch of the pons
TS19: midbrain	Present	Regionally restricted	5F	Expression at the midbrain/hindbrain junction is in the pia matter and in small patch of the pons

Structure	Level	Pattern	Image	Note
TS20: outflow tract	Present		4D
TS20: face mesenchyme	Present	Regionally restricted	4D	Expression is in mesenchyme, particularly in facial mesenchyme.
TS20: aorta-gonad-mesonephros	Present		4D
TS20: nervous system	Absent		4D	There is one small patch of expression in the pons.
TS20: liver	Present		4D

Structure	Level	Pattern	Image	Note
TS20: face mesenchyme	Present	Regionally restricted	6B	Expression is in mesenchyme, particularly in facial mesenchyme.
TS20: aorta-gonad-mesonephros	Present		6B

Structure	Level	Pattern	Image	Note
TS20: outflow tract	Present		6D
TS20: heart ventricle	Present	Regionally restricted	6D	Expression is in the ventricular wall and some of the ventricular cushions.

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Present		6F

Structure	Level	Pattern	Image	Note
TS22: lower jaw mesenchyme	Present		4F
TS22: upper jaw mesenchyme	Present		4F
TS22: embryo mesenchyme	Present	Regionally restricted	4F	Expression is in mesenchyme surrounding pia matter, in the vertebrae and in the herniated gut.

Structure	Level	Pattern	Image	Note
TS22: lower jaw mesenchyme	Present		5H
TS22: upper jaw mesenchyme	Present		5H
TS22: embryo mesenchyme	Present	Regionally restricted	5H	Expression is in mesenchyme surrounding pia matter, in the vertebrae and in the herniated gut.

Structure	Level	Pattern	Image	Note
TS22: lower jaw mesenchyme	Present	Regionally restricted	6H	There is high expression along the bottom of the lower jaw where whisker roots have formed.