Gene Expression Data
Assay Details
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Assay
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Reference:
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J:58079
Barbacci E, et al., Variant hepatocyte nuclear factor 1 is required for visceral endoderm specification. Development. 1999 Nov;126(21):4795-805
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1346715
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Gene symbol:
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Lamc1
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Gene name:
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laminin, gamma 1
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Antibody:
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D18
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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Embyos for this assay were fixed in 3% formaldehyde for 5 minutes.
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Results
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Specimen
Laminin +/-:
embryonic day 5.5; Hnf1btm1Sce/Hnf1b +
(more  )
Specimen
Laminin +/-:
(close  )
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Genetic Background:
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either: (involves: 129P2/OlaHsd) or (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * C57BL/6J * DBA/2)
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Mutant Allele(s):
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Hnf1btm1Sce/Hnf1b+
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Age:
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embryonic day 5.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
Laminin -/-:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd) or (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * C57BL/6J * DBA/2)
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Mutant Allele(s):
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Hnf1btm1Sce/Hnf1btm1Sce
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Age:
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embryonic day 5.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS7: extraembryonic component |
Present |
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4D
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Staining is heterogeneous and sparse indicating the presence of an abnormal basal membrane structure.
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Analysis Tools
