Gene Expression Data
Assay Details
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Assay
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Reference:
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J:41540
Rossel M, et al., Mice mutant for both Hoxa1 and Hoxb1 show extensive remodeling of the hindbrain and defects in craniofacial development. Development. 1999 Nov;126(22):5027-40
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1346704
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Gene symbol:
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Nefm
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Gene name:
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neurofilament, medium polypeptide
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Results
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Specimen
6A:
(close  )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Hoxa1tm1Mrc/Hoxa1+
Hoxb1tm3Mrc/Hoxb1+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: rhombomere 2 |
Present |
Single cells |
6A
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The first branchiomotorneurons to differentiate (identified by neurofilament labeling) are seen in rhombomeres 2 and 4, where they send their fasciculated axons towards their respective exit points.
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| TS15: rhombomere 4 |
Present |
Single cells |
6A
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The first branchiomotorneurons to differentiate (identified by neurofilament labeling) are seen in rhombomeres 2 and 4, where they send their fasciculated axons towards their respective exit points.
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| TS15: rhombomere 3 |
Trace |
Single cells |
6A
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At this stage, almost no neurons and axons expressing the neurofilament protein are observed in r3 and r5.
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| TS15: rhombomere 5 |
Trace |
Single cells |
6A
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At this stage, almost no neurons and axons expressing the neurofilament protein are observed in r3 and r5.
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Specimen
6B:
embryonic day 9.5; Hoxa1tm1Mrc/ Hoxa1tm1Mrc, Hoxb1tm3Mrc/Hoxb1 +
(more  )
Note: These mutants have lost the characteristic hindbrain organization into distinct even- and odd-numbered rhombomeres.
Specimen
6B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Hoxa1tm1Mrc/Hoxa1tm1Mrc
Hoxb1tm3Mrc/Hoxb1+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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These mutants have lost the characteristic hindbrain organization into distinct even- and odd-numbered rhombomeres.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Single cells |
6B
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No region of low neuron content is observed in this mutant. Rhombomere 2 neurons are still recognizable in the mutant because of the convergence of axons towards an exit point. Branchiomotor neurons are present, sending out axons all along the neural tube
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Specimen
6C:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Hoxa1tm1Mrc/Hoxa1tm1Mrc
Hoxb1tm3Mrc/Hoxb1tm3Mrc
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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These mutants have lost the characteristic hindbrain organization into distinct even- and odd-numbered rhombomeres.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Single cells |
6C
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No region of low neuron content is observed in this mutant. Rhombomere 2 neurons are still recognizable in the mutant because of the convergence of axons towards an exit point. Branchiomotor neurons are present, sending out axons all along the neural tube
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Analysis Tools