Antibody:	Cdx1-specific antibody (NH2)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS11: extraembryonic visceral endoderm	Present	Regionally restricted	4A , 4B , 4C	Expression was detected in the nuclei of ecto- and mesodermal cells of the visceral endoderm in the area of the primitive streak with the highest intensity being found in the ectodermal cells.
TS11: embryo	Present	Regionally restricted	4C , 4B	The expression domain was restricted to the immediate region of the primitive streak, with a slight lateral extension within the ectoderm.
TS11: primitive streak	Present		4C , 4B
TS11: embryo ectoderm	Present		4C

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	4D	Protein distribution extended anterior to the node in ectodermal cells. The anterior boundary of expression in the ectoderm corresponded with the level of the preotic sulcus.
TS11: embryo mesoderm	Present	Regionally restricted	4D	Protein distribution extended anterior to the node in mesodermal cells. Expression in the mesoderm showed a slightly more posterior boundary than ectodermal cells.
TS11: primitive streak	Present	Regionally restricted	4E	There was a broadening of protein distribution in the region of the primitive streak.
TS11: embryo	Present	Regionally restricted	4E	The expression domain extended to the whole posterior area.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	4F	The anterior expression boundary in the neural tissue was localized within the posterior hindbrain.
TS12: head somite	Present	Uniform	4F
TS12: trunk somite	Present	Uniform	4F

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	4G	The anterior expression boundary in the neural tissue was localized within the posterior hindbrain.
TS13: somite	Present		4G

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	4I , 4H	The anterior expression boundary in the neural tissue was localized within the posterior hindbrain.
TS13: migrating neural crest	Present	Regionally restricted	4H , 4I	Expression was present in the dorsal margin of the neural folds when emigration of the first neural crest cells takes place.
TS13: neural fold	Present	Regionally restricted	4H , 4I	Expression was present in the dorsal margin of the neural folds when emigration of the first neural crest cells takes place.
TS13: somite 01	Trace		4I
TS13: somite 02	Trace		4I
TS13: somite 03	Trace		4I
TS13: somite 04	Trace		4I
TS13: trunk somite	Present		4I

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		5A	Expression could be observed along the entire length of the neural tube.
TS15: future hindbrain	Present	Single cells	5A	Neural crest cells which seem to leave the neural tube to occupy a dorsolateral position represented the most anterior expressing cells in the posterior hindbrain of the embryo.
TS15: trunk somite	Present	Regionally restricted	5A	The first 14 of 24 somites show a concentration of protein in their dorsal part. The more posterior somites maintain an equal protein distribution.
TS15: forelimb bud	Present		5A	Immunostaining visualized an even protein distribution along the anterior-posterior and the proximal-distal axis of the forelimb bud, while the dorsal part exhibited a slightly higher protein concentration.

Structure	Level	Pattern	Image	Note
TS16: embryo	Present	Single cells	5B	Expression could be detected in cells that seem to leave the neural tube to occupy a dorsolateral position.
TS16: trunk somite	Present	Regionally restricted	5B	Expression was concentrated in the dorsal part of anterior somites, while the posterior somites maintained an equal protein distribution.

Structure	Level	Pattern	Image	Note
TS15: trunk somite	Present	Regionally restricted	5C	The first 14 of 24 somites show a concentration of protein in their dorsal part. The more posterior somites maintain an equal protein distribution.
TS15: trunk mesenchyme	Present	Regionally restricted	5C	Expression was detected in the intermediate mesoderm situated between the paraxial mesoderm and the somatopleure or splanchnopleure between the level of the seventh and the eleventh somite.
TS15: mesonephric tubule	Weak		5C

Structure	Level	Pattern	Image	Note
TS14: trunk somite	Present	Regionally restricted		The first 5 of 16 somites show a concentration of protein in their dorsal part. The more posterior somites maintain an equal protein distribution.

Structure	Level	Pattern	Image	Note
TS17: trunk somite	Present	Regionally restricted	5E	Concentration of expression in the dorsal somitic edge seemed to be restricted to the first 16 to 17 somites. Posterior to this position, expression decreased and increased slightly in the area of the hindlimb bud (24 to 30 somite).
TS17: nephric duct	Present		5E

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Graded	5F	Expression was distributed evenly along the anterior-posterior and the proximal-distal axis of the forelimb bud, while the dorsal part exhibited slightly higher expression.

Structure	Level	Pattern	Image	Note
TS12: embryo ectoderm	Present	Regionally restricted	6A	The protein distribution extended anterior to the node in ectodermal and mesodermal cells. The anterior boundary of expression in the ectoderm corresponded with the level of the preotic sulcus.
TS12: embryo mesenchyme	Present	Regionally restricted	6A	The protein distribution extended anterior to the node in ectodermal and mesodermal cells. Expression in the mesoderm showed a slightly more posterior boundary than that in the ectoderm.

Structure	Level	Pattern	Image	Note
TS12: embryo ectoderm	Present	Regionally restricted	6B	The protein distribution extended anterior to the node in ectodermal and mesodermal cells. The anterior boundary of expression in the ectoderm corresponded with the level of the preotic sulcus.
TS12: embryo mesenchyme	Present	Regionally restricted	6B	The protein distribution extended anterior to the node in ectodermal and mesodermal cells. Expression in the mesoderm showed a slightly more posterior boundary than that in the ectoderm.

Structure	Level	Pattern	Image	Note
TS12: notochord	Absent		6C/D
TS12: embryo endoderm	Absent		6C/D

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	7A	The major location of neural crest cells in a more ventral position exhibited no significant expression.
TS15: trunk dermomyotome	Present	Graded	7A	Strongest expression occurred in the dorsal part of the dermomyotome, while the ventral area exhibited reduced expression.
TS15: trunk sclerotome	Absent		7A
TS15: forelimb bud mesenchyme	Present		7A
TS15: forelimb bud ectoderm	Absent		7A

Structure	Level	Pattern	Image	Note
TS17: surface ectoderm	Present	Single cells	7B
TS17: forelimb bud	Strong		7B
TS17: neural tube	Weak	Regionally restricted	7B	The neural tube expression decreased. The major location of neural crest cells in a more ventral position exhibited no significant expression.
TS17: trunk somite	Weak		7B

Structure	Level	Pattern	Image	Note
TS20: tail somite	Present	Regionally restricted	7C	Expression was detected exclusively in the dorsal somitic edge of the myotome.
TS20: hindlimb	Weak	Graded	7C	The hindlimb bud showed a similar pattern of expression to the forelimb at day 9.5, with an even protein distribution along the anterior-posterior and the proximal-distal axis, while the dorsal part exhibited a slightly higher protein concentration.

Structure	Level	Pattern	Image	Note
TS20: nephric duct	Present		7D