Probe:	Cdx-1 probe1
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		1A/B	Transcripts were present in ectodermal and mesodermal cells of the primitive streak.
TS11: embryo mesoderm	Weak	Regionally restricted	1A/B	Anterior to the node, expression extended into the neurectodermal and paraxial mesodermal cells with a boundary directly rostral to the notochordal cells.
TS11: neural ectoderm	Weak	Regionally restricted	1A/B	Anterior to the node, expression extended into the neurectodermal and paraxial mesodermal cells with a boundary directly rostral to the notochordal cells.
TS11: notochordal process	Absent		1A/B	Notochordal cells showed no expression.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		1C/D	Transcripts were present in ectodermal and mesodermal cells of the primitive streak.
TS11: embryo mesoderm	Weak	Regionally restricted	1C/D	Anterior to the node, expression extended into the neurectodermal and paraxial mesodermal cells with a boundary directly rostral to the notochordal cells.
TS11: neural ectoderm	Weak	Regionally restricted	1C/D	Anterior to the node, expression extended into the neurectodermal and paraxial mesodermal cells with a boundary directly rostral to the notochordal cells.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Regionally restricted	1E/F	Expression was restricted to the ingressing zone at the anterior end but became progressively broader towards the posterior end of the embryo.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Regionally restricted	1G/H	Expression was restricted to the ingressing zone at the anterior end but became progressively broader towards the posterior end of the embryo.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Regionally restricted	1I/J	Expression was restricted to the ingressing zone at the anterior end but became progressively broader towards the posterior end of the embryo.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present		2A/B
TS12: embryo	Present	Regionally restricted	2A/B	Expression extended laterally to the primitive streak. Expression anterior to the node decreased strongly.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted	2C/D	Expression could be found in the whole posterior part of the embryo.
TS13: future hindbrain	Present	Regionally restricted	2C/D	Expression was detected in the neurectoderm at the posterior hindbrain level.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	2E/F	Expression was detected in the neurectoderm at the posterior hindbrain level.
TS13: primitive streak	Present		2E/F
TS13: embryo mesenchyme	Weak	Regionally restricted	2E/F	The mesoderm exhibited a slightly weaker expression compared to the ectodermal cells in the primitive streak reagion. The signal was reduced further in mesodermal cells located anterior to the node, than in ectodermal cells of the developing neural tube.
TS13: neural tube	Present	Regionally restricted	2E/F	Expression was detected in ectodermal cells of the developing neural tube.
TS13: somite	Present		2E/F

Structure	Level	Pattern	Image	Note
TS15: trunk somite	Present	Regionally restricted	2G/H	Expression became restricted to the dorsal somitic edge of the anterior trunk somites.
TS15: forelimb bud	Present	Uniform	2G/H