Probe:	Dan probe1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: head mesenchyme	Present		5A	Expression is apparent in the mesodermal wings that underlie the anterior neural plate.

Structure	Level	Pattern	Image	Note
TS11: head mesenchyme	Present		5B	Expression is apparent in the mesodermal wings that underlie the anterior neural plate.

Structure	Level	Pattern	Image	Note
TS12: head paraxial mesenchyme	Present		5C	Expression was seen in the paraxial mesoderm both within and rostral to the somitic column.

Structure	Level	Pattern	Image	Note
TS12: head paraxial mesenchyme	Present		5D	Expression was seen in the paraxial mesoderm both within and rostral to the somitic column.

Structure	Level	Pattern	Image	Note
TS13: trunk somite	Present	Graded	5E	Expression was strongest throughout the two most caudal somites but appeared to be rapidly downregulated as these somites matured.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted		More rostrally, a second wave of expression appeared to border the neural tube.

Structure	Level	Pattern	Image	Note
TS15: trunk dermomyotome	Present	Regionally restricted	5F	Expression was clearly restricted to the dermamyotome of somites along the entire body axis.
TS15: forelimb bud mesenchyme	Present	Regionally restricted	5F	Expression was seen in the outer mesenchymal layers of the dorsal and venetral bud and in an anterior proximal domain.

Structure	Level	Pattern	Image	Note
TS17: tail dermomyotome	Present		5G
TS17: trunk dermomyotome	Present		5G
TS17: forelimb bud mesenchyme	Present	Regionally restricted	5G	Expression remained restricted to the outer dorsal and ventral mesenchyme.

Structure	Level	Pattern	Image	Note
TS15: trunk dermomyotome	Present	Regionally restricted	5H	Expression was clearly restricted to the dermamyotome of somites along the entire body axis.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	5I	Expression remained restricted to the outer dorsal and ventral mesenchyme.

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Strong		5J
TS15: olfactory placode	Strong	Regionally restricted	5J	Expression was observed at the edge of the olfactory pit.

Structure	Level	Pattern	Image	Note
TS17: embryo	Strong	Regionally restricted	5K	The entire distal facial primorida stained strongly