Gene Expression Data
Assay Details
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Assay
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Reference:
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J:32712
Biggiogera M, et al., Nucleolar distribution of proteins B23 and nucleolin in mouse preimplantation embryos as visualized by immunoelectron microscopy. Development. 1990 Dec;110(4):1263-70
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1344970
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Gene symbol:
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Npm1
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Gene name:
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nucleophosmin 1
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Antibody:
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anti-B23 antibody
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Detection system:
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Secondary antibody coupled to colloidal gold
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Results
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Specimen
not shown:
embryonic day 0.5
(more  )
Note: Zygote.
Specimen
not shown:
(close  )
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Genetic Background:
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(C57BL/6 x DBA/2)F1
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Age:
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embryonic day 0.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Zygote.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
Regionally restricted |
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The prenucleolar masses were labelled with the anti-B23 antibody. The gold grains are regularly scattered on the compact nucleolus. The nucleoplasm is not significantly labelled.
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Specimen
3:
embryonic day 1.0
(more )
Note: 2-cell stage.
Specimen
3:
(close )
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Genetic Background:
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(C57BL/6 x DBA/2)F1
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Age:
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embryonic day 1.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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2-cell stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Weak |
Regionally restricted |
3
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The number of gold grains observed over the compact fibrillar mass is considerably lower than in the zygote.
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Specimen
5:
embryonic day 2.0
(more )
Note: 4-8 cell stage.
Specimen
5:
(close )
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Genetic Background:
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(C57BL/6 x DBA/2)F1
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Age:
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embryonic day 2.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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4-8 cell stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-8 cell stage embryo |
Present |
Regionally restricted |
5
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The gold grains are present on the still condensed area as well as on the nucleolonemal network. Labelling is present on the granular component and on the dense fibrillar component. Fibrillar centers are never labelled.
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Specimen
not shown:
embryonic day 3.0
(more )
Note: Morula.
Specimen
not shown:
(close )
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Genetic Background:
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(C57BL/6 x DBA/2)F1
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Age:
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embryonic day 3.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Morula.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
Regionally restricted |
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B23 and nucleolin co-localize on dense fibrillar components and granular components. The residual condensed core masses are virtually unlabelled.
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Specimen
not shown:
embryonic day 3.0
(more )
Note: Early blastocyst.
Specimen
not shown:
(close )
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Genetic Background:
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(C57BL/6 x DBA/2)F1
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Age:
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embryonic day 3.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Early blastocyst.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Strong |
Regionally restricted |
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The nucleolus displays no more condensed areas and is heavily labelled with both B23 and nucleolin. They co-localize on the dense fibrillar components and the granular components, the fibrillar centers being devoid of labelling.
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