Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:13029
Haack H, et al., The establishment of murine Hox-1 expression domains during patterning of the limb. Dev Biol. 1993 Jun;157(2):410-22
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1344743
|
|
Gene symbol:
|
Hoxd10
|
|
Gene name:
|
homeobox D10
|
|
Probe:
|
pbH5.3-125RV
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
|
Results
|
Specimen
5C, 4.5:
embryonic day 9.75
(more  )
Specimen
5C, 4.5:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.75
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Present |
Regionally restricted |
5C
|
Expression is in a small ventral, distal region. Posterior expression is slightly more extended. Expression is similar to Hoxa10 expression.
|
Specimen
5H, 4.5:
embryonic day 10.5
(more )
Specimen
5H, 4.5:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
5H
|
Expression extends anteriorly compared to TS16, but remains in the ventral half of the limb. Expression is similar to Hoxa10 and extends more proximally than Hoxa11.
|
Specimen
5L, 4.5:
embryonic day 10.5
(more )
Specimen
5L, 4.5:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
5L
|
Expression extends anteriorly compared to TS16, but remains in the ventral half of the limb. Expression is similar to Hoxa10 and extends more proximally than Hoxa11.
|
Specimen
5P, 4.5:
embryonic day 11.0
(more )
Specimen
5P, 4.5:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Present |
Regionally restricted |
5P
|
Expression extends more proximally than in earlier stages.
|
Specimen
5T, 4.5:
embryonic day 11.5
(more )
Specimen
5T, 4.5:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
5T
|
Expression extends further proximally than at TS18. There is enhanced expression in the domain that overlaps Hoxa11 expression.
|
|
Analysis Tools