Gene Expression Data
Assay Details
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Assay
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Reference:
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J:32896
Sundin OH, et al., Region-specific expression in early chick and mouse embryos of Ghox-lab and Hox 1.6, vertebrate homeobox-containing genes related to Drosophila labial. Development. 1990 Jan;108(1):47-58
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1344633
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Gene symbol:
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Hoxa1
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Gene name:
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homeobox A1
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Probe:
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Hox1.6 probe3
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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A similar hybridization pattern was observed with antisense probe from the protein coding region (MGI:1344616).
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Results
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Specimen
5I:
embryonic day 7.5
(more  )
Specimen
5I:
(close  )
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Genetic Background:
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involves: C57BL/6J * CF-1 * SJL/J
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Strong |
Regionally restricted |
5I
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Grains were detected over the primitive streak almost to the tip of the egg cylinder.
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| TS11: extraembryonic component |
Present |
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5I
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The most posterior signal was detected about 5 cell diameters past the amnion in the extraembryonic mesoderm.
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| TS11: notochordal process |
Absent |
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5I
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Since grains were never observed at the tip of the egg cylinder, it is presumed that the head process does not synthesize this message.
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Specimen
7A:
embryonic day 7.5
(more )
Specimen
7A:
(close )
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Genetic Background:
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involves: C57BL/6J * CF-1 * SJL/J
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Strong |
Regionally restricted |
7A
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Grains were detected over the primitive streak almost to the tip of the egg cylinder.
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Specimen
not shown:
embryonic day 7.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: C57BL/6J * CF-1 * SJL/J
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7C:
embryonic day 8.0
(more )
Specimen
7C:
(close )
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Genetic Background:
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involves: C57BL/6J * CF-1 * SJL/J
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: head somite |
Strong |
Regionally restricted |
7C
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Silver grains were observed over the primitive streak and over the somites up to a point near the posterior limit of the foregut pocket.
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| TS12: primitive streak |
Strong |
Regionally restricted |
7C
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Silver grains were observed over the primitive streak and over the somites up to a point near the posterior limit of the foregut pocket.
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