Probe:	Hoxc8 probe5
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Present	Graded	3E	pv12 (T5) was usually the most anterior position for detection of transcripts. Labelling was progressively reduced over more posterior prevertebrae.

Structure	Level	Pattern	Image	Note
TS20: lung	Absent		4E
TS20: pharynx	Absent		4E
TS20: trachea	Absent		4E

Structure	Level	Pattern	Image	Note
TS20: heart	Absent
TS20: outflow tract aortic component	Absent
TS20: pharynx	Absent			Expression was not detected in ventral pharynx.
TS20: lung	Absent
TS20: stomach	Absent
TS20: thyroid gland	Absent
TS20: trachea	Absent
TS20: mesonephros	Present
TS20: metanephros	Present

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Regionally restricted	6F	The anterior boundary lay within the spinal cord and was at the level of the 5th prevertebra in the ventral part of the cord and the 3rd prevertebra in the middle of the cord. Irregularities in the border are consistent among different embryos.
TS20: spinal cord	Present	Regionally restricted	6F	The anterior boundary lay within the spinal cord and was at the level of the 5th prevertebra in the ventral part of the cord and the 3rd prevertebra in the middle of the cord. Irregularities in the border are consistent among different embryos.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Graded		A progressive anterior-to-posterior fall in abundance of transcripts was observed.